REFERENCES for LAMBDA MINIFOR bioreactors and laboratory fermentors

Here you will find the latest scientific publications on the LAMBDA MINIFOR laboratory fermentor & bioreactor systems. For more references, see www.lambda-instruments.com/fermenter-bioreactor/#publications, please.

«The best references of a laboratory fermenter & bioreactor system are its successful applications

We at LAMBDA Laboratory Instruments are always happy to receive your publications where the LAMBDA MINIFOR fermenter & bioreactor system support you in your analytical, chemical and life science laboratory projects.

2024: Scientific publications about laboratory fermentor & bioreactor systems

400 ml isolated tomato cells in a LAMBDA MINIFOR bioreactor (20 °C, pH 5.8) got sparged (10 L/h) to achieve varied O2 concentrations (21 kPa, 5 kPa, and 0 kPa) for the study of postharvest losses of fruits and vegetable during controlled atmosphere storage as a result of low O2 stress.

Mahomud, M. S., Islam, M., & Roy, J. (2024). Effect of low oxygen stress on the metabolic responses of tomato fruit cells. Heliyon, e24566.
https://doi.org/10.1016/j.heliyon.2024.e24566

Keywords: Tomato fruit, cell culture, hypoxic stress, anoxic stress, metabolomics


 

Simulated human colon model LAMBDA MINIFOR with basal nutrient medium (37 °C, pH 6.8) and faecal slurries:

Pusuntisumpun, N., Tunsagool, P., Nitisinprasert, S., & Nakphaichit, M. (2024). Impacts of combining Limosilactobacillus reuteri KUB‐AC5 and Limosilactobacillus fermentum KUB‐D18 on overweight gut microbiota using a simulated human colon model. International Journal of Food Science & Technology.
https://doi.org/10.1111/ijfs.16941

Keywords: Gut microbiota modulation, high-fat diet, metabolomic analysis, metagenomic analysis, potential probiotics, Thai overweight

2023: Scientific publications about laboratory fermentor & bioreactor systems

Continuous itaconic acid (IA) fermentations with A. terreus spp.: LAMBDA MINIFOR 3L autoclavable bench-top laboratory fermenter (working volume 1.8 litres; 4 days batch, then dilution rate 0.007 h-1; 33 °C; controlled low pH; one week 1.5 vvm air, then 0.2 vvm O2) with septum for sterile sampling and LAMBDA FiSH-TAIL agitator (3.0 s-1) providing gentle and efficient mixing for optimal oxygenation and gas exchange in absence of air flooding which are advantageous properties when working with filamentous microorganisms to achieve clump growth as the most efficient morphology for IA-production.

Hülberné Beyer, É. A., Nemestóthy, N., & Bélafiné Bakó, K. (2023). Case Study of Continuous Itaconic Acid Fermentation by Aspergillus Terreus in a Bench-Scale Bioreactor. Hungarian Journal of Industry and Chemistry, 51(2), 57-63.
https://doi.org/10.33927/hjic-2023-19

Pictures of morphology during fermentation: Fig. 1 on https://hjic.mk.uni-pannon.hu/index.php/hjic/article/view/1214/1092 (2024 Feb. 08)

Keywords: bipolar membrane electrodialysis, clump morphology, integrated system, oxygen uptake rate


 

Simultaneous saccharification (enzymatic hydrolysis with cellulase and β-glucosidase) and fermentation (Lacticaseibacillus rhamnosus) of pretreated delignified lignocellulosic plum biomass to lactic acid in a LAMBDA MINIFOR Advanced Kit bioreactor (1.7 L; 37 °C /44 °C; pH 5.5 / 6.5; 72 h).

Senila, L., Cadar, O., Kovacs, E., Gal, E., Dan, M., Stupar, Z., Simedru, D., Senila, M. & Roman, C. (2023). L-Poly(lactic acid) Production by Microwave Irradiation of Lactic Acid Obtained from Lignocellulosic Wastes. Int. J. Mol. Sci. 2023, 24, 9817.
https://doi.org/10.3390/ijms24129817

Keywords: L-polylactic acid; renewable biomass; simultaneous saccharification and fermentation; lactic acid


 

Evaluation of viability and maximum specific growth rate of Bacillus licheniformis in a bench-top LAMBDA MINIFOR 7L laboratory bioreactor (2 litres working volume, 37 °C, pH 6.5, 200 rpm, controlled oxygen, submerged batch fermentation (SMF), addition of sterilized Antifoam 204 agent (Sigma-Aldrich))

Dumitru, M. & Ciurescu, G. (2023). Optimization of the fermentation conditions and survival of Bacillus licheniformis as freeze-dried powder for animal probiotic applications. Scientific Papers. Series D. Animal Science. Vol. LXVI, No. 2, 2023; ISSN 2285-5750; ISSN CD-ROM 2285-5769; ISSN Online 2393-2260; ISSN-L 2285-5750.
www.animalsciencejournal.usamv.ro/pdf/2023/issue_2/Art10.pdf (2024 Jan. 02)

Keywords: animal nutrition, Bacillus, bioreactor, freeze-drying, probiotics


 

Bacillus megaterium fermentation performed in bioreactor LAMBDA MINIFOR (1.7 L)

Senila, L., Gál, E., Kovacs, E., Cadar, O., Dan, M., Senila, M. & Roman, C. (2023). Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581. Polymers. 2023, 15, 4488.
https://doi.org/10.3390/polym15234488

Keywords: poly(3-hydroxybutyrate); microwave irradiation; lignocellulosic waste; Bacillus megaterium ATCC 14581


 

Optimization of lipase-catalyzed hydrolysis of vegetable oils: Control and monitoring by a LAMBDA MINIFOR bioreactor (pH, temperature, reaction time, enzyme load and oil/aqueous ratio of the reaction mixture).

Faillace, E., Brunini-Bronzini de Caraffa, V., Mariani, M., Berti, L., Maury, J. & Vincenti, S. (2023). Optimizing the First Step of the Biocatalytic Process for Green Leaf Volatiles Production: Lipase-Catalyzed Hydrolysis of Three Vegetable Oils. International Journal of Molecular Sciences. 2023; 24(15):12274.
https://doi.org/10.3390/ijms241512274

Keywords: lipase; hydrolysis; sunflower oil; hempseed oil; linseed oil; response surface methodology


 

Simulation of in-vitro gut model with LAMBDA MINIFOR 0.3L: Batch fermentations under anaerobic conditions (37 °C, pH 6.8–6.9, 24 h) of human fecal slurry (1% (v/v)) to evaluate the effect of Triphala extracts (1 mg/mL) co-fermentation on microbiota and metabolic changes.

https://doi.org/10.1016/j.jtcme.2023.02.011 graphical abstract

Kwandee, P., Somnuk, S., Wanikorn, B., Nakphaichit, M. & Tunsagool, P. (2023). Efficacy of Triphala extracts on the changes of obese fecal microbiome and metabolome in the human gut model. Journal of Traditional and Complementary Medicine, Volume 13, Issue 2, 2023, Pages 207-217, ISSN 2225-4110,
https://doi.org/10.1016/j.jtcme.2023.02.011

Keywords of publications: T. bellirica, T. chebula, P. emblic, Feces, Metabolome, Microbiome, Obesity, Triphala extracts


 

Gastrointestinal Simulation Model SHIME with LAMBDA MINIFOR 0.3L reactors

Each Simulator of Human Intestinal Microbial Ecosystem consists of five reactors, mimicking different sections of the human Gastrointestinal tract.

The video www.youtube.com/watch?v=hXcpa0bXu6Q shows Assoc. Prof. Massalin Nakphaichit in front of the SHIME reactors
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University.

More about the topic: www.lambda-instruments.com/news/detail/lambda-minifor-multistage-fermenter-for-simulating-the-human-intestinal-microbial-ecosystem-shime/


 

In a bench-scale LAMBDA MINIFOR 7L bioreactor with programmable probes, RODMs (a set of organisms with novel metabolism, efficiently degrading highly-concentrated aromatics) were developed for a high-density microbial ecosystem.

Reference: Ahmad, M., Yousaf, M., Han, J.-C., Huang, Y., Zhou, Y. & Tang, Z. (2023). Development of Biocatalytic Microbial Ecosystem (FPUS@RODMs@In-PAOREs) for Rapid and Sustainable Degradation of Various Refractory Organics. Journal of Hazardous Materials, 2023, 131514, ISSN 0304-3894, https://doi.org/10.1016/j.jhazmat.2023.131514Ahmad, M., Yousaf, M., Han, J.-C., Huang, Y., Zhou, Y. & Tang, Z. (2023). Development of Biocatalytic Microbial Ecosystem (FPUS@RODMs@In-PAOREs) for Rapid and Sustainable Degradation of Various Refractory Organics. Journal of Hazardous Materials, 2023, 131514, ISSN 0304-3894,
https://doi.org/10.1016/j.jhazmat.2023.131514

Keywords: Industrial wastewater; Refractory organics; Biocatalysis; Bio-carrier; Enzymatic catalysis

www.lambda-instruments.com/news/detail/creating-a-microbial-ecosystem-in-the-bio-carrier-harnessing-the-power-of-the-lambda-minifor-biorea/

 


Hybridoma cell (inoculation 4.0×10E5 cells/mL (90% viability)): Fed-batch in a LAMBDA MINIFOR stirred-tank bioreactor.

Llamo, A., Hernández, D., García, C., González, M., Ferro, W., Garay, H., Diago, D., Fajardo, A., Espinosa, L., Padilla, S., Gómez, L., Chinea, G. & and Valdés, R. (2023). Gamma-Immunoglobulin Response Characterization, in COVID-19 Convalescent Patients, Against the Spike Protein S2 Subunit with Eight Linear Peptides for Monoclonal Antibody Generation. BioProcess J, 2023; 22.
https://doi.org/10.12665/J22OA.Llamo

Keywords: SARS-CoV-2, spike protein S2 subunit, IgG, Hybridoma cells, fed-batch


 

Milk was pasteurized at 70 °C for 30 min in LAMBDA MINIFOR fermenters
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LAMBDA MINIFOR fermenters: Temperature effect tests on growth of yeast Kazachstania unispora (initial ~10E6 CFU/ml) in milk (800 ml, ~6 % lactose): from 5 °C to 40 °C (5, 10, 15, 20, 25, 27, 30, 32, 35, 37 and 40 °C) at pH 5.6 (automatic adjustment with 2 M NaOH) and 240 rpm, until stationary phase was reached (inline near-infrared turbidity sensor Optek FC20- ASD10-N)
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LAMBDA MINIFOR fermenters for co-culture experiments at 25 °C of Lacticaseibacillus casei and Kazachstania unispora in modified MRS media as well as in mare milk (initial: ~10E6 CFU/ml, pH = 6.8)
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LAMBDA MINIFOR fermenters for co-culture experiments at 30 °C of Lactobacillus kefiri and Kazachstania unispora in modified MRS media as well as in mare milk (initial: ~10E6 CFU/ml, pH = 6.8)

Kondybayev, A., Achir, N., Mestres, C., Collombel, I., Strub, C., Grabulos, J., Akhmetsadykov, N., Aubakirova, A., Kamidinkyzy, U., Ghanmi, W. & Konuspayeva, G. (2023). Growth Kinetics of Kazachstania unispora and Its Interaction with Lactic Acid Bacteria during Qymyz Production. Fermentation 2023, 9, 101.
https://doi.org/10.3390/fermentation9020101

Keywords: Kazachstania unispora; volatile organic compounds; mare milk; solid-phase microextraction; mathematical modeling


 

LAMBDA MINIFOR 0.4L bioreactor for medium conditioning (37 °C, pH 7.2, constant anaerobic conditions (10% H2, 10% CO2, N2)) with weighing module and medium pumps (30 ml/h) in a dynamic in vitro biofilm model for mimicking the oral cavity environment.

Alonso-Español, A., Bravo, E., Ribeiro-Vidal, H., Virto, L., Herrera, D., Alonso, B. & Sanz, M. (2023). The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces. Int. J. Mol. Sci. 2023, 24, 2335. https://doi.org/10.3390/ijms24032335Alonso-Español, A., Bravo, E., Ribeiro-Vidal, H., Virto, L., Herrera, D., Alonso, B. & Sanz, M. (2023). The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces. Int. J. Mol. Sci. 2023, 24, 2335. https://doi.org/10.3390/ijms24032335

Keywords: curcumin; xanthohumol; peri-implantitis; oral biofilms; scanning electron microscopy; confocal laser microscopy; polymerase chain reaction; in vitro; antibacterial; antibiofilm

© 2023 by the authors (see above). This picture is an open access picture distributed under the terms and conditions of the Creative Commons Attribution (CC BY)


Itaconic acid (IA) production by continuous aerobic fungal fermentation (1.8 L, 37 °C, 0.2 vvm pure oxygen, pH naturally reduced to <2.5) in a LAMBDA MINIFOR bioreactor with native itaconic acid overporducing Aspergillus terreus NRRL 1966 using high glucose concentration (maintained at ~150 g/L) as carbon source.

Rózsenberszki, T., Komáromy, P., Hülber-Beyer, E., Pesti, A., Koók, L., Bakonyi, P., Bélafi-Bakó, K. & Nemestóthy, N. (2023). Bipolar membrane electrodialysis integration into the biotechnological production of itaconic acid: A proof-of-concept study. Chemical Engineering Research and Design, Volume 190, 2023, Pages 187-197, ISSN 0263-8762.  https://doi.org/10.1016/j.cherd.2022.12.023Rózsenberszki, T., Komáromy, P., Hülber-Beyer, E., Pesti, A., Koók, L., Bakonyi, P., Bélafi-Bakó, K. & Nemestóthy, N. (2023). Bipolar membrane electrodialysis integration into the biotechnological production of itaconic acid: A proof-of-concept study. Chemical Engineering Research and Design, Volume 190, 2023, Pages 187-197, ISSN 0263-8762.
https://doi.org/10.1016/j.cherd.2022.12.023

Keywords: Itaconic acid, C5H6O4, Fermentation broth, Bipolar membrane, Electrodialysis, EDBM, Crystallization, Fermentation temperature, Mass transfer mechanism

© 2023 by the authors (see above). This picture is an open access picture distributed under the terms and conditions of the Creative Commons Attribution (CC BY)

 

2022: Scientific publications about laboratory fermentor & bioreactor systems

For releasing bioactive peptides, diluted Kiwicha protein isolate (KPI) from Amaranthus caudatus L. seed flour was subjected to enzymatic hydrolysis with Bioprotease LA-660 using a LAMBDA MINIFOR bioreactor for continuous stirring, pH- and temperature control (pH 8; 50 °C).

Martinez-Lopez, A., Rivero-Pino, F., Villanueva, A., Toscano, R., Grao-Cruces, E., Marquez-Paradas, E., Martin, M.E., Montserrat-de la Paz, S. & Millan-Linaresa, M.C. (2022). Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway. Food & Function 2022 Oct 21.;
https://doi.org/10.1039/D2FO02177C

Keywords: protein hydrolysates, Amaranthus caudatus L., Bioprotease LA-660, NLRP3 inflammasome pathway, CACO-2 cell line, cytokines, functional food ingredients


 

Production of biosurfactant (BS, 32 g/L ) by Lactobacillus acidophilus in a laboratory bioreactor LAMBDA MINIFOR 7L (3.5 litres working volume, 30 °C, pH = 6.5, 120 h batch culture), purfication of supernatant and BS studies.

Abdullah, A.R. & Ismail, H.H.(2022). Cytotoxic effect of biosurfactant produced by Lactobacillus acidophilus and study its synergistic effect with certain antibiotics against S. aureus and E. coli. Eurasian Medical Research Periodical, 12, 33–40.
www.geniusjournals.org/index.php/emrp/article/view/2162 (2022 Sept. 22)

Keywords: Biosurfactant, bioreactor, emuslification, chromatography, production parameters, batch culture experiment, cytotoxin


 

Continuous culture: 1 month chemostat in LAMBDA MINIFOR PBR photobioreactor
(1L; 30 °C; 1 L/min of CO2-enriched (0.5%) air; 5 Hz, pH 8; white light) with Synechocystis sp. PCC 6803

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Berwanger, L.C., Burmester, J., Yao, L., Brandt, D., Busche, T., Kalinowski, J., Hudson, E.P., Ebenhöh, O., Axmann, I.M. & Machné, R. (2022). Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis. Nucleic Acids Research, Volume 50, Issue 22, 9 December 2022, Pages 12790–12808.
https://doi.org/10.1093/nar/gkac1132

Keywords: Synechocystis, cyanobacteria, topoisomerase I, DNA supercoiling, gyrase subunits


 

Biocatalytic resolution of lupanine racemate in industrial wastewater by Pseudomonas putida LPK411 using a lab‐scale bioreactor LAMBDA MINIFOR 0.4L under batch operation.

Parmaki, S., Esteves, T., Gonçalves, J.M.J. Catenacci, A., Malpei, F., Ferreira, F.C., Afonso C.A.M & Koutinas, M. (2022). Selective microbial resolution of lupanine racemate: Bioprocess development and the impact of carbon catabolite repression on industrial wastewater valorisation. Biomass Conv. Bioref. (2022).
https://doi.org/10.1007/s13399-022-03383-3

Keywords in publication: Lupanine, Chiral resolution, Fed-batch, Enantiomeric excess, Pseudomonas putida LPK411, Gene expression


 

Escherichia coli (E. coli; E44Δ) mutant strain for production of large quantity of outer membrane vesicles (OMVs) in a LAMBDA MINIFOR 7L fermenter

Allahghadry, T., Bojesen, A.M., Whitehead, B.J. and Antenucci, F. (2022). Clarification of large-volume bacterial cultures using a centrifuge-free protocol. J Appl Microbiol. Accepted Author Manuscript. https://doi.org/10.1111/jam.15608

Keywords: bacterial filtration system; large-volume filtration; centrifuge-free filtration, filter aid


 

During 65 days, two continuous (HRT= 5 days) stirrer tank fermenters LAMBDA MINIFOR were operated under anaerobic conditions (N2 into headspace & sparging), each with 1 liter working volume (modification of lactate / acetate concentrations) inoculated with caproate-producing sludge (Caproiciproducens genus (Ruminococcaceae family)), temperature control (30 °C, build-in IR heater, Mettler InPro 3253 probe) and pH control (pH 5.5, NaOH 2M, HCl 0.5M) ) with four peristaltic pumps (feed, effluent, base & acid) and daily liquid sampling for carboxylates and alcohols analysis.

Brodowski, F., Lezyk, M., Gutowska, N., Kabasakal, T. & Oleskowicz-Popiel, P. (2022). Influence of lactate to acetate ratio on biological production of medium chain carboxylates via open culture fermentation. Science of The Total Environment, Volume 851, Part 1, 2022, 158171, ISSN 0048-9697. https://doi.org/10.1016/j.scitotenv.2022.158171

Keywords: Caproate; Chain elongation; Caproiciproducens; Lactate; Acetate; anaerobe fermentation; continuous fermentation


 

Continuous anaerobic fermentation during 140 days in LAMBDA MINIFOR fermentors https://doi.org/10.1016/j.scitotenv.2021.149885

LAMBDA MINIFOR fermenters with weighing modules to control the harvest pumps for the continuous anaerobic biotechnological process were used to verify how the external acetate affects the product spectrum, gas production, stability and efficiency of carboxylates production.

Brodowski, F., Lezyk, M., Gutowska & Oleskowicz-Popiel, P. (2022). Effect of external acetate on lactate-based carboxylate platform: Shifted lactate overloading limit and hydrogen co-production. Science of The Total Environment, Volume 802, 2022, 149885, ISSN 0048-9697. https://doi.org/10.1016/j.scitotenv.2021.149885

Keywords: Chain elongation; Carboxylate platform; Caproate Hydrogen; Lactate overloading; Acetate; continuous culturing; anaerobic biotechnological process

Lactic acid bacteria growth experiments: Effect of temperature on Lacticaseibacillus casei and Lactobacillus kefiri.

Kondybayev, A.; Konuspayeva, G.; Strub, C.; Loiseau, G.; Mestres, C.; Grabulos, J.; Manzano, M.; Akhmetsadykova, S. & Achir, N. (2022). Growth and Metabolism of Lacticaseibacillus casei and Lactobacillus kefiri Isolated from Qymyz, a Traditional Fermented Central Asian Beverage. Fermentation 2022, 8, 367. https://doi.org/10.3390/fermentation8080367

Keywords: lactic acid bacteria qymyz (koumiss) mathematical modeling mare milk

2021: Scientific publications about laboratory fermentor & bioreactor systems

The growth, glucose consumption & ethanol production of Saccharomyces cerevisiae LM strain in synthetic broth were modeled for the most important intrinsic. LAMBDA MINIFOR 1L fermenters equipped with a cold water condenser on air exit pipes (LAMBDA Instruments GmbH, Baar- Switzerland) were used.

Kouamé, C., Loiseau, G., Grabulos, J., Boulanger, R. & Mestres, C. (2021). Development of a model for the alcoholic fermentation of cocoa beans by a Saccharomyces cerevisiae strain. International Journal of Food Microbiology, Volume 337, 2021, 108917, ISSN 0168-1605. https://doi.org/10.1016/j.ijfoodmicro.2020.108917

Keywords: Yeast, Starter, Chocolate, Gamma concept, Predictive microbiolog

Continuous culture of cyanobacteria Synechocystis sp. PCC 6803 in a LAMBDA MINIFOR 1L PBR photo-bioreactor

Illustration by HHU Düsseldorf, L. Goldschmidt, www.biorxiv.org/content/10.1101/2021.07.26.453758v2.full#F15

Illustration by HHU Düsseldorf, L. Goldschmidt, www.biorxiv.org/content/10.1101/2021.07.26.453758v2.full#F15

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Brandt, D., Busche, T., Kalinowski, J., Ebenhöh, O., Axmann, I. M. & Machné, R. (2021) Uncoupling of the Diurnal Growth Program by Artificial Genome Relaxation in Synechocystis sp. PCC 6803. bioRxiv 2021.07.26.453758.
https://doi.org/10.1101/2021.07.26.453758

 Keywords: photobioreactor, laboratory, Synechocystis sp. PCC 6803, cyanobacteria, growth, model microorganism, DNA supercoiling, artificial genome relaxation, gyrase, topoisomerase I

Experiments on liquid phase (hemicelluloses hydrolysate) for xylitol production: The fermentation of 250 ml of detoxified hydrolysate was conducted in a 1L fermenter (LAMBDA MINIFOR bench-top-laboratory-fermenter) and pH adjustment (pH 5.0) at aerobic conditions at 30 °C for 60 h.

Shalsh, D., Nagimm, D., Alrheem, M.A. & Alrheem, S.A. (2021). Batch fermentation and Simultaneous Saccharification and Fermentation (SSF) processes by Meyerozyma Guilliermondii Strain F22 and Saccharomyces cerecvisae for xylitol and bioethanol co-production. Al-Qadisiyah Journal of Pure Science, 26(4), 80–94. https://doi.org/10.29350/qjps.2021.26.4.1347

Keywords: Bioethanol, xylitol, rice straw, bioreactor, Meyerozyma Guilliermondii, Saccharomyces cerecvisae

The hydrolysis of kiwicha protein isolate (KPI) is performed under continuous stirring, using a LAMBDA MINIFOR fermenter-bioreactor, at controlled conditions of pH and temperature: Bioprotease LA-660 is added at a ratio enzyme/substrate = 0.3 AU/g protein (pH 8) for 5, 10, 15, 30, and 60 min.

Paz, S.M.-d.l.; Martinez-Lopez, A.; Villanueva-Lazo, A.; Pedroche, J.; Millan, F. & Millan-Linares, M.C. (2021). Identification and Characterization of Novel Antioxidant Protein Hydrolysates from Kiwicha (Amaranthus caudatus L.). Antioxidants 10, no. 5: 645; https://doi.org/10.3390/antiox10050645

Keywords in publication: kiwicha; protein hydrolysate; bioactive compound; food ingredient; antioxidant activity.

The biological transformation of white sorghum biomass was performed under operating conditions similar to the MixAlco process in the LAMBDA MINIFOR bioreactor.

Shalsh, F.J., Alrazzaq, N.A., Nagimm D.K., Alrheem, M.A., Alrheem S.A. & Abd-alalah, K. (2021). Bioconversion of white sorghum biomass using MixAlco fermentation process. DYSONA – Applied Science. 2021(2), 21-27. ISSN 2708-6283. https://doi.org/10.30493/DAS.2021.248966

Keywords: Fermentation, Carboxylate platform, Carboxylic acids, Bioethanol, Volatile matter

LAMBDA MINIFOR bioreactor and fermentor for schooling at university

Technologische Fachoberschule Bruneck, Fachrichtung Chemie, Werkstoffe und Biotechnologie (2021). Bioreaktor. https://www.facebook.com/watch/?v=239416121364132 (2022 September 13)

Keywords: TFO Bruneck, bioreactor, fermentor, technical equipment

Bioreactor system LAMBDA MINIFOR as an artificial mouth system for the growth of biofilm

LA BOCA ARTIFICIAL DE DENTAID UNA REVOLUCIÓN EN INVESTIGACIÓN BUCODENTAL DENTAID EXPERTISE.  PUBLICACIÓN PARA PROFESIONALES DE LA ODONTOLOGÍA, NÚM. 18.
https://aprenderly.com/doc/3463742/la-boca-artificial-de-dentaid-una-revoluci%C3%B3n-en-investiga%E2%80%A6?page=5 (2022 September 13)

2020: Scientific publications about laboratory fermentor & bioreactor systems

LAMBDA MINIFOR bioreactor used to produce biotechnologically itaconic acid from glucose by Aspergillus terreus fungal strain: 1.8 L medium with 120 g/L glucose as substrate, batch mode, aerobe: aeration 2 L/min (STP) = 6.5 mg/L inital DO, 37 °C, 2 Hz agitation, pH 3)

Nemestóthy, N., Komáromy, P., Bakonyi, P. et al. (2020). Carbohydrate to Itaconic Acid Conversion by Aspergillus terreus and the Evaluation of Process Monitoring Based on the Measurement of CO2 Waste and Biomass. Valorization 2020. https://doi.org/10.1007/s12649-019-00729-3

Keywords: Itaconic acid, Aspergillus terreus, Glucose, Process monitoring, Off-gas analysis

LAMBDA MINIFOR bioreactor (equipped with 2 peristaltic pumps (saliva and permeate pump), an overhead mixer, a redox-temperature-pH probe, a temperature control unit and a submerged hollow fibre membrane module) is used as a Rumen membrane bioreactor to produce volatile fatty acids (VFA) from crop residues (lignocellulosic biomass) by mimicking the digestive system of ruminant animals.

Nguyena, A.Q., Nguyena, L.N., Johira, M.A.H., Ngoa, H.-H., Chaves, A.V. & Nghiemac, L.D. (2020). Derivation of volatile fatty acid from crop residues digestion using a rumen membrane bioreactor: a feasibility study. Bioresource Technology 2020. https://doi.org/10.1016/j.biortech.2020.123571

Keywords: Volatile fatty acids, Lignocellulosic biomass, Anaerobic digestion, Membrane bioreactor, Rumen microbial community

Different yeast strains were cultivated in the fermentor LAMBDA MINIFOR 0.4L to study the metabolic cycle and pathway: A) 200 ml batch: YMC-YE / YMC-MD media, pH 3.5, 0.15 L/min aeration, 30 °C; B) starvation 6 h; C) continuous culture: dilution rate of 0.082 1/h.

Feltham, J.E., Xi, S., Murray, S.C., Wouters, M., Urdiain-Arraiza, J., Heilig, R., George, C., Townley, A.F., Roberts, E., Kessler, B.M., Liberatori, S., Charles, P.D., Angel, A., Fischer, R. & Mellor, J. (2020). Transcriptional changes are regulated by metabolic pathway dynamics but decoupled from protein levels. bioRxiv 833921. https://doi.org/10.1101/833921

Keywords: S. pombe, yeast, metabolic cycles, metabolic pathway studies

Enzymatic hydrolysis experiments were carried out in the lab-scale LAMBDA MINIFOR stirred-batch bioreactor. The pretreated vine-shoot waste was delignified with sodium chlorite for lignin removal and then enzymatically hydrolyzed using new types of enzymes (cellulase from Trichoderma reesei and b-glucosidase).

Kovacs, E., Scurtu, D.A., Senila, L., Cadar, O., Dumitras, E.D. & Roman, C. (2020). Green Protocols for the Isolation of Carbohydrates from Vineyard Vine-Shoot Waste. Analytical Letters. https://doi.org/10.1080/00032719.2020.1721001

Keywords: Carbohydrates, chlorite delignification, microwave pretreatment, structural characterization, vine-shoot waste

2019 - 2003: Scientific publications about laboratory fermentor & bioreactor systems

pH optimization for the aerobical production of itaconic acid catalyzed by Aspergillus terreus in a LAMBDA MINIFOR bioreactor: Batch, working volume 1.8 L, medium with 120 g/L glucose substrate, 37 °C, pH 3 - pH 2.5 - pH 4 - pH 3 - pH 2.5, stirrer 2 Hz, aeration 1.5 L (STP)/min.

Komáromy, K., Bakonyi, P., Kucska, A., Tóth, G., Gubicza, L., Bélafi-Bakó, K. & Nemestóthy, N. (2019). Optimized pH and Its Control Strategy Lead to Enhanced Itaconic Acid Fermentation by Aspergillus terreus on Glucose Substrate. Fermentation 2019, 5(2), 31. https://doi.org/10.3390/fermentation5020031

Keywords: itaconic acid, A. terreus, pH control, glucose, kinetic analysis, Gompertz-model

LAMBDA MINIFOR 0.4L bioreactor with PRECIFLOW medium pumps used in turbidostat experiments with recombinant cells in continuous culture operation mode.

Pasotti, L., Bellato, M., Politi, N., Casanova, M., ucca, S., Gabriella, M., De Angelis, C. & Magni, P. (2019). A synthetic close-loop controller circuit for the regulation of an extracellular molecule by engineered bacteria. IEEE Trans Biomed Circuits Syst. 2019 Feb; 13(1):248-258. https://doi.org/10.1109/TBCAS.2018.2883350

Author Keywords: Biological system modelling, feedback circuits, in vivo, synthetic biology, systems biology, MG1655-Z1 strain

Lab-Scale Production of Rhamnolipid by Pseudomonas Aeruginosa A3 using MINIFOR Benchtop Bioreactor.

Faqri, A. F., Hayder, N.H. & Hashim, A.J. (2019). Lab-scale production of Rhamnolipid by Pseudomonas Aeruginosa A3 and study its synergistic effect with certain antibiotics against some pathogenic bacteria. Iraqi Journal of Agricultural Sciences –2019:50(5):1290-1301. https://doi.org/10.13140/RG.2.2.10802.35520

Keywords: biosurfactant, bioreactor, emuslification, chromatography

Study of the effect of sugar starvation at various O2 conditions on isolated cells from leafy vegetables on Lambda Minifor. 2017:

Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress. Baiye Mfortaw Mbong Victor, Jerry Ampofo-Asiama, Maarten Hertog, Annemie H Geeraerd and Bart M. Nicolai. Postharvest Biology and Technology. Volume 126, April 2017, Pages: 23-33.  Department of Biochemistry, University of Cape Coast, Ghana. Keywords: Lamb’s lettuce (Valerianella locusta L.) cells; Sugar starvation; Low O2 stress; Metabolic response; Metabolite profiling; 13C labelling.

Abstract: Sugar starvation is a common phenomenon occurring in most leafy vegetables after harvest and storage. Additionally, leafy vegetables are subjected to low O2 stress when stored in modified atmosphere conditions. In this study, the metabolism of isolated lamb’s lettuce cells was studied upon sugar starvation under O2 stress conditions, using 13C labelled glucose. Fast depletions of the soluble sugars were observed, being more pronounced under aerobic conditions than under low O2 stress conditions. Sugar starvation under aerobic conditions resulted in increased levels and decreased 13C label incorporation of TCA cycle intermediates and amino and fatty acids originating from glycolytic and TCA cycle pathways, compared to starving cells incubated under low O2stress. On incubation under low O2 stress a switch in metabolism from aerobic to fermentation metabolism was observed. Under low O2 stress conditions, increased levels and 13C label incorporated in hexose phosphates, pyruvate, lactate, GABA, alanine, together with increased levels of acetaldehyde, ethanol and ethyl acetate was observed indicating fermentative metabolism was triggered.

Robust cellulosic ethanol production from sugarcane bagasse with Saccharomyces cerevisiae ATCC 20602 in LAMBDA MINIFOR laboratory bioreactor under aerobic and anaerobic conditions with controlled redox potential measurement: 2016.

Abstract: The present study deals with the production of cellulosic ethanol from bagasse using the synthesized TiO2 coupled nanocellulose (NC-TiO2) as catalyst. Aspergillus nidulans AJSU04 cellulase was used for the hydrolysis of bagasse. NC-TiO2 at various concentrations was added to bagasse in order to enhance the yield of reducing sugars. Complex interaction between cellulase, bagasse, NC-TiO2 and the reaction environment is thoroughly studied. A mathematical model was developed to describe the hydrolysis reaction. Ethanol production from enzymatically hydrolyzed sugarcane bagasse catalyzed with NC-TiO2 was carried out using Saccharomyces cerevisiae ATCC 20602. The glucose release rates and ethanol concentrations were determined. Ethanol produced was found to be strongly dependent on pretreatment given, hydrolysis and fermentation conditions. The study confirmed the promising accessibility of NC-TiO2, for enhanced glucose production rates and improved ethanol yield.

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S. pyogenes Cas9 protein expressed using a 3L computer-controlled MINIFOR bioreactor in batch medium followed by exponential feeding: 2015.

Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins.Ménoret, Séverine, Anne De Cian, Laurent Tesson, Séverine Remy, Claire Usal, Jean-Baptiste Boulé, Charlotte Boix et al.  Scientific reports5 (2015). 2015; 7 Oct 2015. Nature ublishing Group. INSERM UMR 1064-ITUN; CNRS UMS3556 Nantes; CNRS UMR7196; Sorbonne Universities; University Pierre & Marie Curie; France. 

Abstract: The generation of genetically-modified organisms has been revolutionized by the development of new genome editing technologies based on the use of gene-specific nucleases, such as meganucleases, ZFNs, TALENs and CRISPRs-Cas9 systems. The most rapid and cost-effective way to generate genetically-modified animals is by microinjection of the nucleic acids encoding gene-specific nucleases into zygotes. However, the efficiency of the procedure can still be improved. In this work we aim to increase the efficiency of CRISPRs-Cas9 and TALENs homology-directed repair by using TALENs and Cas9 proteins, instead of mRNA, microinjected into rat and mouse zygotes along with long or short donor DNAs. We observed that Cas9 protein was more efficient at homology-directed repair than mRNA, while TALEN protein was less efficient than mRNA at inducing homology-directed repair. Our results indicate that the use of Cas9 protein could represent a simple and practical methodological alternative to Cas9 mRNA in the generation of genetically-modified rats and mice as well as probably some other mammals.

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Fermentation of engineered microorganism in laboratory scale bioreactor MINIFOR for efficient conversion of lactose-to-ethanol: 2015.

"Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology."Pasotti, L., S. Zucca, M. Casanova, N. Politi, I. Massaiu, G. Mazzini, G. Micoli, C. Calvio, M. G. Cusella De Angelis, and P. Magni. In Engineering in Medicine and Biology Society (EMBC), 2015 37th Annual International Conference of the IEEE, pp. 953-956. IEEE, 2015. Department of Electrical, Computer & Biomedical Engineering and Interdepartmental Research Centre for Tissue Engineering, University of Pavia, Italy. Keywords: lactose-to-ethanol conversion; microorganism optimization; synthetic biology; whey protein; permeate; pollutant waste disposal; genetic optimization; green energy production; biofuel production; cheese production process; dairy waste; biocatalyst

 

Abstract: Whey is an abundant by-product of cheese production process and it is considered a special waste due to its high nutritional load and hypertrophic potential. Technologies for whey valorization are available. They can convert such waste into high-value products, like whey proteins. However, the remaining liquid (called permeate) is still considered as a polluting waste due to its high lactose concentration. The alcoholic fermentation of lactose into ethanol will simultaneously achieve two important goals: safe disposal of a pollutant waste and green energy production. This methodology paper illustrates the workflow carried out to design and realize an optimized microorganism that can efficiently perform the lactose-to-ethanol conversion, engineered via synthetic biology experimental and computational approaches.

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Six-species flow cell biofilm model was developed by culturing bacteria in LAMBDA MINIFOR Bioreactor to evaluate the biofilm development under flow and shear conditions: 2015.

The use of in vitro model systems to study dental biofilms associated with caries: a short review. Salli, Krista M., and Arthur C. Ouwehand.Journal of oral Microbiology 7 (2015). J Oral Microbiol. 2015; 7: 10.3402/jom.v7.26149. DuPont Nutrition and Health, Kantvik Active Nutrition, Finland. Keywords: dental caries, batch culture, continuous culture, artificial mouth, flow cell, microcosm

 

Abstract: A dental biofilm forms a distinct environment where microorganisms live in a matrix of extracellular polysaccharides. The biofilm favors certain bacteria and creates a habitat that functions differently compared to planktonic bacteria. Reproducible model systems which help to address various questions related to biofilm formation, the process of caries development, and its prevention are needed and are continuously developed. Recent research using both batch culture, continuous culture and flow cells in caries biofilm formation is presented. The development of new techniques and equipment has led to a deeper understanding of how caries biofilms function. Biofilm models have also been used in the development of materials inhibiting secondary caries. This short review summarizes available models to study these questions.

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Quantification of ribosomal proteins (RPs) from Yeast cells cultured in MINIFOR and mouse embryonic stem cells (ESC) to study the core RPs stoichiometry: 2015.

Differential stoichiometry among core ribosomal proteins, arXiv:1406.0399 Nikolai Slavov, Stefan Semrau, Edoardo Airoldi, Bogdan Budnik, Alexander van Oudenaarden, , Jun 2015. Cell Reports 13 , 865–873 November 3, 2015. Harvard University, USA; Broad Institute of MIT and Harvard, USA and Hubrecht Institute, Netherlands . Keywords: Budding Yeast cells, Embryonic stem cells (ESC), Ribosomal Protein, RP, ribosomes, mRNA, mass-spectrometry, posttranslational modification, PTM).

Abstract: Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.

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Growing yeast cultures (DBY12007) in MINIFOR at steady state to study the aerobic glycolysis and energy flux: 2014.

Constant Growth Rate Can Be Supported by Decreasing Energy Flux and Increasing Aerobic Glycolysis. Nikolai Slavov, Bogdan A. Budnik, David Schwab, Edoardo M. Airoldi, and Alexander van Oudenaarden, Cell Reports 7, 705–714, May 8, 2014   Massachusetts Institute of Technology, USA; Harvard University, USA; Hubrecht Institute, Netherlands and Princeton University, USA. Keywords: Yeast, aerobic glycolysis, exponential growth, O2 consumption, CO2 production, amino acids, mRNAs, proteins, posttranslational modifications, stress sensitivity, respiratory quotient (RQ).

Abstract: Fermenting glucose in the presence of enough oxygen to support respiration, known as aerobic glycolysis, is believed to maximize growth rate. We observed increasing aerobic glycolysis during exponential growth, suggesting additional physiological roles for aerobic glycolysis. We investigated such roles in yeast batch cultures by quantifying O2 consumption, CO2 production, amino acids, mRNAs, proteins, posttranslational modifications, and stress sensitivity in the course of nine doublings at constant rate. During this course, the cells support a constant biomass-production rate with decreasing rates of respiration and ATP production but also decrease their stress resistance. As the respiration rate decreases, so do the levels of enzymes catalyzing rate-determining reactions of the tricarboxylic-acid cycle (providing NADH for respiration) and of mitochondrial folate-mediated NADPH production (required for oxidative defense). The findings demonstrate that exponential growth can represent not a single metabolic/physiological state but a continuum of changing states and that aerobic glycolysis can reduce the energy demands associated with respiratory metabolism and stress survival.

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Development of biofilm model (S. oralis, A. naeslundii, V. parvula, F. nucleatum, A. actinomycetemcomitans and P. gingivalis) in continuous flow in a LAMBDA MINIFOR 0.4L bioreactor.

Soto, I. S. (2013). Desarrollo del modelo de boca artificial en flujo continuo en el biorreactor Lambda Minifor. Universidad Complutense de Madrid Master en Ciencias Odontológicas.
https://docta.ucm.es/rest/api/core/bitstreams/39d8318b-a49b-4cb3-a164-e4faab18f52b/content (2024 Feb. 05)

Keywords: biofilm, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Veillonella parvula, Actinomyces naeslundii, Porphyromonas gingivalis, Streptococcus oralis

LAMBDA MINIFOR bioreactor to grow the oral bacteria (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis) under planktonic conditions: 2014.

Abstract: Bacteria in the oral cavity grow in the form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. The aims of this study were: (i) to develop and to characterize an in-vitro biofilm model with oral bacteria growing under flow and shear conditions; and (ii) to demonstrate the usefulness of the model for evaluating the activity of three antiplaque agents.We used a bioreactor to grow the oral bacteria Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis under planktonic conditions. Biofilms were established using a modified Robbins device on hydroxyapatite (HAP) discs. Three- to 7-d-old biofilms were analysed using culture methods, scanning electron microscopy, Live/Dead staining and fluorescence in-situ hybridization (confocal laser scanning microscopy). Finally, we assessed the antimicrobial activity of three mouthrinses [0.12% chlorhexidine (CHX), 0.12% chlorhexidine and sodium fluoride (CHX+NaF) and 0.12% chlorhexidine and 0.05% cetylpyridinium chloride (CHX+CPC)] using a planktonic test (short interval–killing test) and in our 4-d biofilm model. The viable cell counts showed that each species was consistently found in the biofilms throughout the study. The architecture and cell distribution were similar to those described for biofilms in situ, with the exception of a thin layer of living cells that was found close to the HAP. The effectiveness test of the mouthwashes demonstrated that cells in biofilms showed more tolerance compared with planktonic cells. Moreover, it was observed that in 4-d biofilm formed in vitro, CHX+CPC caused significantly higher mortality compared with CHX (p = 0.003) and CHX+NaF (p < 0.001). Our results suggest that we have a highly reproducible system for multispecies oral biofilm formation and that it is a useful tool for assessing antibacterial molecules before their clinical evaluation. It also has great potential to be used in basic research on supragingival and subgingival biofilms.

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Cultivation of microalgae (Chlorella vulgaris Beyerinck) in laboratory bioreactor MINIFOR: 2014.

Mikrovetikate Chlorella vulgaris kasvatamise eelkatsed bioreaktoris Lambda Minifor
Heitur, Heiko. "Mikrovetika Chlorella vulgaris Beyerincki kasvatamine CO2 sidumise eesmärgil." PhD diss., 2014. Estonian University of Life Sciences, Estonia. 

 

 

Selective and non-selective batch fermentation of date extract using Saccharomyces cerevisiae (commercial strain used in bakeries (wild strain), glucose selective strains ATCC 36858 and ATCC 36859) studied in LAMBDA MINIFOR fermentor: 2014.

Selective fermentation of pitted dates by S. cerevisiae for the production of concentrated fructose syrups and ethanol,  Meilana Dharma Putra, Ahmed E. Abasaeed, Mohamed A. Zeinelabdeen, Mohamed H. Gaily, Ashraf K. Sulieman, Journal of Physics: Conference Series 495 (2014) 012034  Chemical Engineering Department, King Saud University, Saudi Arabia. Keywords: Selective, non-selective, fermentation, yeast, S. cerevisiae, fructose, ethanol, date, HPLC, kinetic profile, batch.

Abstract: About half of worldwide production of dates is unconsumed. Dates contain over 75 % reduced sugars (mostly glucose and fructose with nearly equal amount). Compared to the commercial Saccharomyces cerevisiae wild strain, the strains ATCC 36858 and 36859 could produce high concentration fructose syrups. The fructose fractions obtained were 95.9 and 97.4% for ATCC 36858 and 86.5 and 91.4% for ATCC 36859 at 30 and 33°C, respectively. Fructose yields higher than 90% were obtained using ATCC 36858 compared to those obtained using ATCC 36859 which were 87.3 and 66.1% at 30 and 33°C, respectively. The ethanol yield using ATCC 36858 was higher than that using ATCC 36859 by 16 and 9% at 30 and 33°C, respectively. Through this finding, the production of fructose and ethanol from date extract is a promising process. Moreover, the fructose fractions obtained here (about 90%) are much higher than those obtained with the commercial process, i.e. 55 % fructose syrups.

Controlled growth of Staphylococcus aureus under various concentrations of BAC (benzalkonium chloride) in MINIFOR fermentor. 2013:

The Role of the qacA Gene in Mediating Resistance to Quaternary Ammonium Compounds. Dana Cervinkova, Vladimir Babak, Durdica Marosevic, Iva Kubikova, and Zoran Jaglic. Microbial Drug Resistance. June 2013, 19(3): 160-167.  Veterinary Research Institute, Brno, Czech Republic. Keywords: Staphylococcus aureus, benzalkonium chloride (BAC), exponential phase, expression, real-time PCR, culture, concentration

 

Abstract: Conditions facilitating resistance to quaternary ammonium compounds (QACs) were investigated in Staphylococcus aureus SK982 exposed to benzalkonium chloride (BAC; a member of QACs) under various circumstances. S. aureus SK982 carrying the qacA gene encoding for resistance to QACs was grown in the presence of stable or gradually increasing concentrations of BAC, or it was exposed to this antiseptic in the exponential phase of growth. Bacteria cultivated in the highest BAC concentrations that did not retard their growth comparing to the untreated control were subjected to real-time quantitative polymerase chain reaction analysis for relative expression of the efflux genes qacA and norA. Under such conditions, S. aureus SK982 tolerated a relatively low stable concentration of BAC (1.22 mg/L) when compared with a gradually increasing antiseptic concentration (tolerance of 4.88 mg/L). However, in both cases, qacA expression was not significant. The culture exposed in the exponential phase of growth tolerated the highest concentration of BAC (9.76 mg/L) as also accompanied by significant overexpression of qacA. Expression of norA was relatively low regardless of the conditions tested. It seems that under the short-term conditions, the phase of bacterial growth is more important for the expression of BAC resistance than the capability to adapt to this antiseptic. This study provides a deeper insight into the relevance of the qac genes in conferring resistance to QACs.

Systems for High-Density Hybridoma Growth and High-yield mAb production in cell culture: Bench-top stirred tank bioreactors, 1-5 L (MINIFOR - LAMBDA Laboratory Instruments). 2013:

Abstract: Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science—both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications... From the eradication of smallpox to combating cancer, antibodies present an attractive solution to a range of biomedical problems. They are relatively easy to make and use, have great flexibility in applications, and are cost effective for most labs. This volume will assist biomedical researchers and students and pave the way for future discovery of new methods for making and using antibodies for a host of applications.

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The metabolic stress response of tomato cell culture (Lycopersicum esculentum) to low oxygen studied using LAMBDA MINIFOR Bioreactor. 2013:

The metabolic response of cultured tomato cells to low oxygen stress.  Ampofo-Asiama, J., Baiye, V. M. M., Hertog, M. L. A. T. M., Waelkens, E., Geeraerd, A. H., Nicolai, B. M. (2013), Plant Biology. German Botanical Society and The Royal Botanical Society of the Netherlands. Keywords: 3C label; cell culture; low O2 stress; Lycopersicum esculentum; metabolome

Abstract: The storage of fruits and vegetables under a controlled atmosphere can induce low oxygen stress, which can lead to post-harvest losses through the induction of disorders such as core breakdown and browning. To gain better understanding of the metabolic response of plant organs to low oxygen, cultured tomato cells (Lycopersicum esculentum) were used as a model system to study the metabolic stress response to low oxygen (0 and 1 kPa O2). By adding 13C labelled glucose, changes in the levels of polar metabolites and their 13C label accumulation were quantified. Low oxygen stress altered the metabolite profile of tomato cells, with the accumulation of the intermediates of glycolysis in addition to increases in lactate and sugar alcohols. 13C label data showed reduced label accumulation in almost all metabolites except lactate and some sugar alcohols. The results showed that low oxygen stress in tomato cell culture activated fermentative metabolism and sugar alcohol synthesis while inhibiting the activity of the TCA cycle and the biosynthesis of metabolites whose precursors are derived from central metabolism, including fluxes to most organic acids, amino acids and sugars.

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LAMBDA MINIFOR Bioreactor used for recombinant protein (Chemokines) expression in E. coli. 2013:

 

PhD Thesis. 2013. Birgit Kramp, "Establishing the interaction between the CC chemokine ligand 5 and the receptors CCR1 and CCR5
Fakultät für Mathematik, Informatik und Naturwissenschaften, RWTH Aachen, Belgium.

 

Recombinant expression of the Met-CCL5, protease resistant CXCL12 (S4V) and F1-CX3CL1 in E. coli to study their role in Cardiovascular disease (CVD). 2013:

 

PhD Thesis. 2013. Projahn, D., Generation, function and therapeutic application of chemotactic cytokines in cardiovascular diseases
Institut für Molekulare Herz-Kreislaufforschung, Fakultät für Mathematik, Informatik und Naturwissenschaften, RWTH Aachen, Belgium.
Fakultät für Mathematik, Informatik und Naturwissenschaften, RWTH Aachen, Belgium.

 

Expression of Caf1 protein using Escherichia coli strain to study mammalian cell adhesion, shape and number of focal adhesion. 2013:

 

PhD Thesis. 2013. Machado Roque, A. I., Protein scaffolds for cell culture, 2013 , Newcastle University

 

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MINIFOR Bioreactors for stem cells. 2012:

A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cellsShayan, N., Ebrahimi, M., Beiki, B. and Janzamin, E. 2012. BIOTECHNOLOGY LETTERS. Department of Regenerative Medicine and Stem Cells and Developmental Biology, Cell Research Center, Royan Institute for Stem Cell Biology and Technology, Royan Cord Blood Bank, Tehran, Iran. Keywords: Static culture; Suspension bioreactor; Umbilical cord blood; Vertical mixing

Abstract: The proliferation and differentiation characteristics of umbilical cord blood mononuclear cells were examined in a non-rotational suspension bioreactor with a fishtail mixer. The system consisted of a glass vessel, a mixer that moved vertically, a data acquisition and control system to continuously monitor pH, temperature and dissolved O2. The bioreactor provided superior expansion of total HSCs and not total cell number, as well as expression of stemness-related genes which followed with increasing in number of colony-forming cells during 14 days of culture compared to T -lask culture. Vertical agitation thus reduces the total cell number, which may be related to increased shear stress, but has no effect on HSC function.

Effective production of Biobutanol from agricultural waste (giant hogweed, hay). 2012:

Abstract: One of the main concerns regarding extensive production of bio-butanol has been associated with the high costs of the substrate (preparation of  fermentable sugars) and the relatively low tolerance of Clostridia to butanol. In this study a simple, mild approach was tested to obtain fermentable sugars from agricultural waste. Giant hogweed and hay was pre-treated with simple boiling and enzymatically hydrolysed. The results demonstrated that after adaptation of the genus Clostridium bacteria to the new substrate, the growth kinetics and sugar consumption of these bacteria we are similar to the ones obtained in traditional culture media.

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Bioethanol production using Yeast (S. cerevisiae) in LAMBDA MINIFOR Fermenter. 2011:

Effect of wheat gluten proteins on bioethanol yield from grain. Burešová, I., Hřivna, L., Applied Energy Volume 88, Issue 4, April 2011, Pages 1205–1210 Agrotest Fyto, Ltd., Kroměříž, Czech Republic; Mendel University in Brno, Brno, Czech Republic Keywords: Bioethanol; Triticale; Wheat; Gluten; Protein

Abstract: Bioethanol can be used as motor fuel and/or as a gasoline enhancer. A high yield feedstock for bioethanol production is cereal grain. Cereal grains containing less gluten proteins (glutenin and gliadin), but high starch, are favoured by distillers because they increase the bioethanol conversion. The direct effect of wheat gluten proteins on bioethanol yield was studied on triticale grain. Examined triticale Presto 1R.1D5+10-2 and Presto Valdy were developed by introducing selected segments of wheat chromosome 1D into triticale chromosome 1R. Even if the samples analysed in this study do not afford to make definitive assumptions, it can be noticed that in analysed cases the presence of gliadin had more significant effect on investigated parameters than the presence of glutenin. Despite the presence of glutenin subunits did not significantly decrease the investigated parameters – specific weight, Hagberg falling number and starch content in grain met the requirements for grain for bioethanol production – protein content was higher than is optimal. The fermentation experiments demonstrated good bioethanol yields but depression in grain yields caused by the presence of wheat gliadin and glutenin decreased the energy balance of Presto Valdy and Presto 1R.1D5+10-2.

Anaerobic fermentation of the glucose component in dates extract by yeast Saccharomyces cerevisiae. 2010:

Abstract: A process comprising of extraction, fermentation, separation and decolorization for the production of high fructose syrups and/or ethanol from dates' extract was developed. In this paper, selective fermentation of the glucose component in dates' extract by Saccharomyces cerevisiae was employed to tailor the composition of the final product (fructose- or ethanol-rich). Over 90% of dates’ sugar was extracted by water. The fermentation experiments were conducted at 30 °C and 120 rpm. The commonly marketed 55% fructose syrup was obtained at two operating conditions, i.e., low (<50%) and high (>92%) sugar conversions. Maximum fructose concentrations (77.2-78.2%) were obtained at moderately high sugar conversions (74-82%). Decolorization by activated carbon for the fructose-rich syrup resulted in 87% of color removal. Preliminary economic analysis revealed the viability of this process.

Anaerobic expression using the LAMBDA MINIFOR. 2007:

Abstract: The genome sequence of the non-sugar-assimilating mesophile Methanococcus maripaludis contains three genes encoding enzymes: a nonphosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR); all these enzymes are potentially capable of catalyzing glyceraldehyde-3-phosphate (G3P) metabolism. GAPOR, whose homologs have been found mainly in archaea, catalyzes the reduction of ferredoxin coupled with oxidation of G3P. GAPOR has previously been isolated and characterized only from a sugar-assimilating hyperthermophile, Pyrococcus furiosus (GAPORPf), and contains the rare metal tungsten as an irreplaceable cofactor. Active recombinant M. maripaludis GAPOR (GAPORMm) was purified from Escherichia coli grown in minimal medium containing 100 μM sodium molybdate. In contrast, GAPORMm obtained from cells grown in medium containing tungsten (W) and W and molybdenum (Mo) or in medium without added W and Mo did not display any activity. Activity and transcript analysis of putative G3P-metabolizing enzymes and corresponding genes were performed with M. maripaludis cultured under autotrophic conditions in chemically defined medium. The activity of GAPORMm was constitutive throughout the culture period and exceeded that of GAPDH at all time points. As GAPDH activity was detected in only the gluconeogenic direction and GAPN activity was completely absent, only GAPORMm catalyzes oxidation of G3P in M. maripaludis. Recombinant GAPORMm is posttranscriptionally regulated as it exhibits pronounced and irreversible substrate inhibition and is completely inhibited by 1 μM ATP. With support from flux balance analysis, it is concluded that the major physiological role of GAPORMm in M. maripaludis most likely involves only nonoptimal growth conditions.

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pH and temperature continuously recorded with the LAMBDA MINIFOR and SIAM software. 2005:

Abstract: The genome sequence of the non-sugar-assimilating mesophile Methanococcus maripaludis contains three genes encoding enzymes: a nonphosphorylating NADP+-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR); all these enzymes are potentially capable of catalyzing glyceraldehyde-3-phosphate (G3P) metabolism. GAPOR, whose homologs have been found mainly in archaea, catalyzes the reduction of ferredoxin coupled with oxidation of G3P. GAPOR has previously been isolated and characterized only from a sugar-assimilating hyperthermophile, Pyrococcus furiosus (GAPORPf), and contains the rare metal tungsten as an irreplaceable cofactor. Active recombinant M. maripaludis GAPOR (GAPORMm) was purified from Escherichia coli grown in minimal medium containing 100 μM sodium molybdate. In contrast, GAPORMm obtained from cells grown in medium containing tungsten (W) and W and molybdenum (Mo) or in medium without added W and Mo did not display any activity. Activity and transcript analysis of putative G3P-metabolizing enzymes and corresponding genes were performed with M. maripaludis cultured under autotrophic conditions in chemically defined medium. The activity of GAPORMm was constitutive throughout the culture period and exceeded that of GAPDH at all time points. As GAPDH activity was detected in only the gluconeogenic direction and GAPN activity was completely absent, only GAPORMm catalyzes oxidation of G3P in M. maripaludis. Recombinant GAPORMm is posttranscriptionally regulated as it exhibits pronounced and irreversible substrate inhibition and is completely inhibited by 1 μM ATP. With support from flux balance analysis, it is concluded that the major physiological role of GAPORMm in M. maripaludis most likely involves only nonoptimal growth conditions.

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Bioreactors - An overview of the innovations implemented in MINIFOR bioreactors. 2003:

Abstract: A major effort has been invested in making bioreactors more efficient. The goal has been achieved in several steps. Firstly, a replacement of the traditional rotation movement by an oscillating up and down movement of stirring discs driven by an electromagnet yields several important advantages. A soft, whole volume movement of the medium results in optimal gas distribution throughout the medium and is also advantageous for cell growth. No vortex is formed and the elimination of baffles simplifies the construction of the bioreactor. A single silicone membrane efficiently replaces traditional seals and assures perfect sterility. In the case of extremely sensitive cells gas distribution tubing can be wound on a spiral fixed to the axis. The up and down movement facilitates the gas transfer and simultaneously provides a gentle movement of the medium. Secondly, a new heat radiation system has also been introduced. A heating spiral in a gilded parabolic reflector has been placed under the bottom of the vessel. The IR rays efficiently heat up the culture without overheating it at any volume of medium. Several innovations allow the construction of high quality bioreactors at lower cost.

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