REFERENCIAS-BIBLIOGRAFÍA fermentador & biorreactor LAMBDA MINIFOR

Ahmad, M., Yousaf, M., Han, J.-C., Huang, Y., Zhou, Y. & Tang, Z. (2023). Development of Biocatalytic Microbial Ecosystem (FPUS@RODMs@In-PAOREs) for Rapid and Sustainable Degradation of Various Refractory Organics. Journal of Hazardous Materials, 2023, 131514, ISSN 0304-3894,
https://doi.org/10.1016/j.jhazmat.2023.131514

Palabras clave: Aguas residuales industriales; Orgánicos refractarios; Biocatálisis; Bioportador; Catálisis enzimática

www.lambda-instruments.com/es/noticias/detail/creating-a-microbial-ecosystem-in-the-bio-carrier-harnessing-the-power-of-the-lambda-minifor-biorea/

Llamo, A., Hernández, D., García, C., González, M., Ferro, W., Garay, H., Diago, D., Fajardo, A., Espinosa, L., Padilla, S., Gómez, L., Chinea, G. & and Valdés, R. (2023). Gamma-Immunoglobulin Response Characterization, in COVID-19 Convalescent Patients, Against the Spike Protein S2 Subunit with Eight Linear Peptides for Monoclonal Antibody Generation. BioProcess J, 2023; 22.
https://doi.org/10.12665/J22OA.Llamo

Palabras clave: SARS-CoV-2, subunidad S2 de la proteína de la espiga, IgG, células de hibridoma, lote alimentado

La leche se pasteurizó a 70 °C durante 30 minutos en fermentadores LAMBDA MINIFOR
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Fermentadores LAMBDA MINIFOR: Pruebas de efecto de la temperatura en el crecimiento de la levadura Kazachstania unispora (inital ~106 CFU-mL-1) en leche (800 ml, ~6% lactosa): de 5 °C a 40 °C (5, 10, 15, 20, 25, 27, 30, 32, 35, 37, y 40 °C) a pH 5.6 (ajuste automático con 2 M NaOH) y 240 rpm, hasta que se alcanzó la fase estacionaria (sensor de turbidez en línea de infrarrojo cercano Optek FC20- ASD10-N).
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Fermentadores LAMBDA MINIFOR para experimentos de cocultivo a 25 °C de Lacticaseibacillus casei y Kazachstania unispora en medios MRS modificados, así como en leche de yegua (inital: ~106 UFC-mL-1, pH = 6,8)
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Fermentadores LAMBDA MINIFOR para experimentos de cocultivo a 30 °C de Lactobacillus kefiri y Kazachstania unispora en medios MRS modificados, así como en leche de yegua (inital: ~106 UFC-mL-1, pH = 6,8).

Kondybayev, A., Achir, N., Mestres, C., Collombel, I., Strub, C., Grabulos, J., Akhmetsadykov, N., Aubakirova, A., Kamidinkyzy, U., Ghanmi, W. & Konuspayeva, G. (2023). Growth Kinetics of Kazachstania unispora and Its Interaction with Lactic Acid Bacteria during Qymyz Production. Fermentation 2023, 9, 101.
https://doi.org/10.3390/fermentation9020101

Palabras clave: Kazachstania unispora; compuestos orgánicos volátiles; leche de yegua; microextracción en fase sólida; modelización matemática

Biorreactor LAMBDA MINIFOR de 0,4 L para acondicionamiento del medio (37 °C, pH 7,2, condiciones anaeróbicas constantes (10% H2, 10% CO2, N2)) con módulo de pesaje y bombas de medio (30 ml/h) en un modelo dinámico de biopelícula in vitro para imitar el entorno de la cavidad oral.

Alonso-Español, A., Bravo, E., Ribeiro-Vidal, H., Virto, L., Herrera, D., Alonso, B. & Sanz, M. (2023). The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces. Int. J. Mol. Sci. 2023, 24, 2335. https://doi.org/10.3390/ijms24032335

Palabras clave: curcumina; xantohumol; periimplantitis; biopelículas orales; microscopía electrónica de barrido; microscopía láser confocal; reacción en cadena de la polimerasa; in vitro; antibacteriano; antibiopelícula

© 2023 by the authors (see above). This picture is an open access picture distributed under the terms and conditions of the Creative Commons Attribution (CC BY)

Producción de ácido itacónico (AI) mediante fermentación fúngica aeróbica continua (1,8 L, 37 °C, 0,2 vvm de oxígeno puro, pH reducido naturalmente a <2,5) en un biorreactor LAMBDA MINIFOR con Aspergillus terreus NRRL 1966, sobreproductor nativo de ácido itacónico, utilizando una alta concentración de glucosa (mantenida en ~150 g/L) como fuente de carbono.

Rózsenberszki, T., Komáromy, P., Hülber-Beyer, E., Pesti, A., Koók, L., Bakonyi, P., Bélafi-Bakó, K. & Nemestóthy, N. (2023). Bipolar membrane electrodialysis integration into the biotechnological production of itaconic acid: A proof-of-concept study. Chemical Engineering Research and Design, Volume 190, 2023, Pages 187-197, ISSN 0263-8762.
https://doi.org/10.1016/j.cherd.2022.12.023

Palabras clave: Ácido itacónico, C5H6O4, Caldo de fermentación, Membrana bipolar, Electrodiálisis, EDBM, Cristalización, Temperatura de fermentación, Mecanismo de transferencia de masa.

Cultivo continuo: 1 mes en quimiostato con el fotobiorreactor LAMBDA MINIFOR PBR (1L; 30 °C; 1 L/min de aire enriquecido con CO2 (0,5%); 5 Hz, pH 8; luz blanca) con Synechocystis sp. PCC 6803

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Berwanger, L.C., Burmester, J., Yao, L., Brandt, D., Busche, T., Kalinowski, J., Hudson, E.P., Ebenhöh, O., Axmann, I.M. & Machné, R. (2022). Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis. Nucleic Acids Research, Volume 50, Issue 22, 9 December 2022, Pages 12790–12808.
https://doi.org/10.1093/nar/gkac1132

Palabras clave: Synechocystis, cianobacterias, topoisomerasa I, superenrollamiento del ADN, subunidades girasa

Resolución biocatalítica de racemato de lupanina en aguas residuales industriales por Pseudomonas putida LPK411 usando un biorreactor a escala de laboratorio LAMBDA MINIFOR de 0.4L en modo de operación discontinuo.

Parmaki, S., Esteves, T., Gonçalves, J.M.J. Catenacci, A., Malpei, F., Ferreira, F.C., Afonso C.A.M & Koutinas, M. (2022). Selective microbial resolution of lupanine racemate: Bioprocess development and the impact of carbon catabolite repression on industrial wastewater valorisation. Biomass Conv. Bioref. (2022).
https://doi.org/10.1007/s13399-022-03383-3

Palabras clave en la publicación: Lupanina, Resolución quiral, Cultivo discontinuo alimentado, Exceso enantiomérico, Pseudomonas putida LPK411, Expresión génica

Para la liberación de péptidos bioactivos, proteina aislada de Kiwicha a partir de harina de semillas de Amaranthus caudatus L. fue sometida a hidrólisis enzimática con Bioproteasa LA-660 utilizando un biorreactor LAMBDA MINIFOR con presión continua, control de pH y temperatura (pH 8; 50 °C).

Martinez-Lopez, A., Rivero-Pino, F., Villanueva, A., Toscano, R., Grao-Cruces, E., Marquez-Paradas, E., Martin, M.E., Montserrat-de la Paz, S. & Millan-Linaresa, M.C. (2022). Kiwicha (Amaranthus caudatus L.) protein hydrolysates reduce intestinal inflammation by modulating the NLRP3 inflammasome pathway. Food & Function 2022 Oct 21.;
https://doi.org/10.1039/D2FO02177C

Palabras clave: hidrolizados de proteínas, Amaranthus caudatus L., Bioproteasa LA-660, vía del inflamasoma NLRP3, línea celular CACO-2, citoquinas, ingredientes alimentarios funcionales

Producción de biosurfactante (BS, 32 g/L) por Lactobacillus acidophilus en un biorreactor de laboratorio LAMBDA MINIFOR 7L (3,5 litros de volumen de trabajo, 30 °C, pH = 6,5, 120 h de cultivo por lotes), purificación del sobrenadante y estudios de BS.

Abdullah, A.R. & Ismail, H.H.(2022). Cytotoxic effect of biosurfactant produced by Lactobacillus acidophilus and study its synergistic effect with certain antibiotics against S. aureus and E. coli. Eurasian Medical Research Periodical, 12, 33–40. www.geniusjournals.org/index.php/emrp/article/view/2162 (2022 Sept. 22)

Palabras clave: Biosurfactante, biorreactor, emulsificación, cromatografía, parámetros de producción, experimento de cultivo por lotes, citotoxina

Biorreactor y fermentador LAMBDA MINIFOR para escuelas y universidades

Technologische Fachoberschule Bruneck, Fachrichtung Chemie, Werkstoffe und Biotechnologie (2021). Bioreaktor.
www.facebook.com/watch/?v=239416121364132  (2022 September 13)

Palabras clave: TFO Bruneck, biorreactor, fermentador, equipo técnico

Experimentos de crecimiento de bacterias del ácido láctico: efecto de la temperatura en Lacticaseibacillus casei y Lactobacillus kefiri

Kondybayev, A.; Konuspayeva, G.; Strub, C.; Loiseau, G.; Mestres, C.; Grabulos, J.; Manzano, M.; Akhmetsadykova, S. & Achir, N. (2022). Growth and Metabolism of Lacticaseibacillus casei and Lactobacillus kefiri Isolated from Qymyz, a Traditional Fermented Central Asian Beverage. Fermentation 2022, 8, 367.
https://doi.org/10.3390/fermentation8080367

Palabras clave: bacterias del ácido láctico kmyz (kumis), modelado matemático, leche de yegua

Durante 65 días, se operaron continuamente dos fermentadores de tanque agitado(HRT= 5 días) LAMBDA MINIFOR en condiciones anaeróbicas (N2 en el espacio de cabeza y rociado), cada uno con un volumen de trabajo de 1 litro (modificación de las concentraciones de lactato / acetato) inoculados con lodo productor de caproato (género Caproiciproducens (familia Ruminococcaceae)), control de temperatura (30 °C, calentador IR incorporado, sonda Mettler InPro 3253) y control de pH (pH 5,5, NaOH 2M, HCl 0,5M)) con cuatro bombas peristálticas (alimentación, efluente , base y ácido) y muestreo líquido diario para análisis de carboxilatos y alcoholes.

Brodowski, F., Lezyk, M., Gutowska, N., Kabasakal, T. & Oleskowicz-Popiel, P. (2022). Influence of lactate to acetate ratio on biological production of medium chain carboxylates via open culture fermentation. Science of The Total Environment, Volume 851, Part 1, 2022, 158171, ISSN 0048-9697.
https://doi.org/10.1016/j.scitotenv.2022.158171

Palabras clave: Caproato; Alargamiento de cadena; caproiciproducens; lactato; Acetato; fermentación anaerobia; fermentación continua

Fermentadores LAMBDA MINIFOR con módulo de pesada para controlar las bombas de cosecha en el proceso biotecnológico anaeróbico continuo para verificar cómo el acetato externo afecta el espectro del producto, la producción de gas, la estabilidad y la eficiencia de producción de carboxilatos.

Brodowski, F., Lezyk, M., Gutowska & Oleskowicz-Popiel, P. (2022). Effect of external acetate on lactate-based carboxylate platform: Shifted lactate overloading limit and hydrogen co-production. Science of The Total Environment, Volume 802, 2022, 149885, ISSN 0048-9697.
https://doi.org/10.1016/j.scitotenv.2021.149885

Palabras clave: Alargamiento de cadena; plataforma de carboxilato; caproato de hidrógeno; sobrecarga de lactato; Acetato; cultivo continuo; proceso biotecnológico anaeróbico

Fermentador LAMBDA MINIFOR de 7L en la producción de un alto volumen de vesículas de membrana externa (OMV) a partir de cepa mutante de Escherichia coli (E. coli; E44Δ).

Allahghadry, T., Bojesen, A.M., Whitehead, B.J. and Antenucci, F. (2022). Clarification of large-volume bacterial cultures using a centrifuge-free protocol. J Appl Microbiol. Accepted Author Manuscript.
https://doi.org/10.1111/jam.15608

Palabras clave: sistema de filtración bacteriana; alto volumen de filtración; filtración sin centrífuga, filtro auxiliar.

La transformación biológica de la biomasa de sorgo blanco se realizó en condiciones de operación similares al proceso MixAlco en el biorreactor LAMBDA MINIFOR.

Shalsh, F.J., Alrazzaq, N.A., Nagimm D.K., Alrheem, M.A., Alrheem S.A. & Abd-alalah, K. (2021). Bioconversion of white sorghum biomass using MixAlco fermentation process. DYSONA – Applied Science. 2021(2), 21-27. ISSN 2708-6283.
https://doi.org/10.30493/DAS.2021.248966

Palabras clave: Fermentación, Plataforma de carboxilatos, Ácidos carboxílicos, Bioetanol, Materia volátil

Se modelaron el crecimiento, consumo de glucosa y producción de etanol de la cepa LM de Saccharomyces cerevisiae en caldo sintético para el intrínseco más importante. Se utilizaron fermentadores LAMBDA MINIFOR de un litro equipados con un condensador de agua fría en tuberías de salida de aire (LAMBDA Instruments GmbH, Baar- Suiza).

Kouamé, C., Loiseau, G., Grabulos, J., Boulanger, R. & Mestres, C. (2021). Development of a model for the alcoholic fermentation of cocoa beans by a Saccharomyces cerevisiae strain. International Journal of Food Microbiology, Volume 337, 2021, 108917, ISSN 0168-1605.
https://doi.org/10.1016/j.ijfoodmicro.2020.108917

Palabras clave: levadura, microorganismo iniciador, chocolate, concepto gamma, microbiología predictiva

Experimentos en fase líquida (hidrolizado de hemicelulosas) para la producción de xilitol: Se realizó la fermentación de 250 ml de hidrolizado detoxificado en un fermentador de 1L (LAMBDA MINIFOR de sobremesa-laboratorio-fermentador) y ajuste de pH (pH 5,0) en condiciones aeróbicas a 30° C durante 60 h.

Shalsh, D., Nagimm, D., Alrheem, M.A. & Alrheem, S.A. (2021). Batch fermentation and Simultaneous Saccharification and Fermentation (SSF) processes by Meyerozyma Guilliermondii Strain F22 and Saccharomyces cerecvisae for xylitol and bioethanol co-production. Al-Qadisiyah Journal of Pure Science, 26(4), 80–94.
https://doi.org/10.29350/qjps.2021.26.4.1347

Palabras clave: Bioetanol, xilitol, paja de arroz, biorreactor, Meyerozyma Guilliermondii, Saccharomyces cerecvisae

La hidrólisis del aislado de proteína de kiwicha (KPI) se realiza bajo agitación continua, utilizando un fermentador-biorreactor LAMBDA MINIFOR, en condiciones controladas de pH y temperatura: Se adiciona Bioproteasa LA-660 a razón enzima/sustrato = 0.3 AU/g proteína ( pH 8) durante 5, 10, 15, 30 y 60 min.

Paz, S.M.-d.l.; Martinez-Lopez, A.; Villanueva-Lazo, A.; Pedroche, J.; Millan, F. & Millan-Linares, M.C. (2021). Identification and Characterization of Novel Antioxidant Protein Hydrolysates from Kiwicha (Amaranthus caudatus L.). Antioxidants 10, no. 5: 645;
https://doi.org/10.3390/antiox10050645

Keywords in publication: kiwicha; protein hydrolysate; bioactive compound; food ingredient; antioxidant activity.

Cultivo continuo de cianobacterias Synechocystis sp. PCC 6803 en un fotobiorreactor LAMBDA MINIFOR 1L

Illustration by HHU Düsseldorf, L. Goldschmidt, www.biorxiv.org/content/10.1101/2021.07.26.453758v2.full#F15

Behle, A., Dietsch, M., Goldschmidt, L., Murugathas, W., Brandt, D., Busche, T., Kalinowski, J., Ebenhöh, O., Axmann, I. M. & Machné, R. (2021) Uncoupling of the Diurnal Growth Program by Artificial Genome Relaxation in Synechocystis sp. PCC 6803. bioRxiv 2021.07.26.453758.
https://doi.org/10.1101/2021.07.26.453758

Palabras clave: fotobiorreactor, laboratorio, Synechocystis sp. PCC 6803, cianobacteria, crecimiento, microorganismo modelo, superenrollamiento de ADN, relajación artificial del genoma, girasa, topoisomerasa I

Experimentos de hidrólisis enzimática se llevaron a cabo en el biorreactor de laboratorio LAMBDA MINIFOR. Los residuos de sarmientos pretratados se deslignificaron con clorito de sodio para eliminar la lignina y luego se hidrolizaron enzimáticamente utilizando nuevos tipos de enzimas (celulasa de Trichoderma reesei y b-glucosidasa).

Kovacs, E., Scurtu, D.A., Senila, L., Cadar, O., Dumitras, E.D. & Roman, C. (2020). Green Protocols for the Isolation of Carbohydrates from Vineyard Vine-Shoot Waste. Analytical Letters.
https://doi.org/10.1080/00032719.2020.1721001

Palabras clave: carbohidratos, deslignificación con cloritos, pretratamiento con microondas, caracterización estructural, residuos de sarmiento

El biorreactor LAMBDA MINIFOR (equipado con 2 bombas peristálticas (bomba de saliva y permeado), un mezclador superior, una sonda de pH-temperatura redox, una unidad de control de temperatura y un módulo de membrana de fibra hueca sumergida) se utiliza como un biorreactor de membrana ruminal para producir compuestos volátiles. ácidos grasos (AGV) a partir de residuos de cultivos (biomasa lignocelulósica) imitando el sistema digestivo de los animales rumiantes.

Nguyena, A.Q., Nguyena, L.N., Johira, M.A.H., Ngoa, H.-H., Chaves, A.V. & Nghiemac, L.D. (2020). Derivation of volatile fatty acid from crop residues digestion using a rumen membrane bioreactor: a feasibility study. Bioresource Technology 2020.
https://doi.org/10.1016/j.biortech.2020.123571

Palabras clave: Ácidos grasos volátiles, Biomasa lignocelulósica, Digestión anaerobia, Biorreactor de membrana, Comunidad microbiana ruminal

Se cultivaron diferentes cepas de levadura en el fermentador LAMBDA MINIFOR 0.4L para estudiar el ciclo metabólico y la ruta: A) lote de 200 ml: medio YMC-YE / YMC-MD, pH 3,5, aireación de 0,15 L/min, 30 °C; B) inanición 6 h; C) cultivo continuo: velocidad de dilución de 0,082 l/h.

Feltham, J.E., Xi, S., Murray, S.C., Wouters, M., Urdiain-Arraiza, J., Heilig, R., George, C., Townley, A.F., Roberts, E., Kessler, B.M., Liberatori, S., Charles, P.D., Angel, A., Fischer, R. & Mellor, J. (2020). Transcriptional changes are regulated by metabolic pathway dynamics but decoupled from protein levels. bioRxiv 833921.
https://doi.org/10.1101/833921

Palabras clave: S. pombe, levadura, ciclos metabólicos, estudios de vías metabólicas

Biorreactor LAMBDA MINIFOR utilizado para producir biotecnológicamente ácido itacónico a partir de glucosa por la cepa fúngica Aspergillus terreus: 1,8 L de medio con 120 g/L de glucosa como sustrato, modo discontinuo, aerobio: aireación 2 L/min (STP) = 6,5 mg/L inicial DO, 37 °C, agitación 2 Hz, pH 3)

Nemestóthy, N., Komáromy, P., Bakonyi, P. et al. (2020). Carbohydrate to Itaconic Acid Conversion by Aspergillus terreus and the Evaluation of Process Monitoring Based on the Measurement of CO2 Waste and Biomass. Valorization 2020.
https://doi.org/10.1007/s12649-019-00729-3

Palabras clave: Ácido itacónico, Aspergillus terreus, Glucosa, Monitoreo de procesos, Análisis de gases de escape

Optimización del pH para la producción aeróbica de ácido itacónico catalizada por Aspergillus terreus en un biorreactor LAMBDA MINIFOR: Lote, volumen de medio 1,8 L, medio que contiene 120 g/L de glucosa, 37°C, pH 3 - pH 2,5 - pH 4 - pH 3 - pH 2,5, agitación 2 Hz, aireación 1,5 L(TPS)/min.

Komáromy, K., Bakonyi, P., Kucska, A., Tóth, G., Gubicza, L., Bélafi-Bakó, K. & Nemestóthy, N. (2019). Optimized pH and Its Control Strategy Lead to Enhanced Itaconic Acid Fermentation by Aspergillus terreus on Glucose Substrate. Fermentation 2019, 5(2), 31
https://doi.org/10.3390/fermentation5020031

Palabras clave: ácido itacónico, A. terreus, control de pH, glucosa, análisis cinético, modelo de Gompertz

Biorreactor LAMBDA MINIFOR 0.4L con bombas de medio LAMBDA PRECIFLOW es utilizado en experimentos turbidostatos con células recombinantes en modo de operación de cultivo continuo.

Pasotti, L., Bellato, M., Politi, N., Casanova, M., ucca, S., Gabriella, M., De Angelis, C. & Magni, P. (2019). A synthetic close-loop controller circuit for the regulation of an extracellular molecule by engineered bacteria. IEEE Trans Biomed Circuits Syst. 2019 Feb; 13(1):248-258.
https://doi.org/10.1109/TBCAS.2018.2883350

Palabras clave del autor: modelado de sistemas biológicos, circuitos de retroalimentación, in vivo, biología sintética, biología de sistemas, cepa MG1655-Z1

Producción de ramnolípido (RL) con Pseudomonas aeruginosa en el fermentador LAMBDA MINIFOR 7L (30 °C, pH 6.5, lote, 120 h) para la purificación del metabolito secundario.

Faqri, A. F., Hayder, N.H. & Hashim, A.J. (2019). Lab-scale production of Rhamnolipid by Pseudomonas Aeruginosa A3 and study its synergistic effect with certain antibiotics against some pathogenic bacteria. Iraqi Journal of Agricultural Sciences –2019:50(5):1290-1301.
https://doi.org/10.13140/RG.2.2.10802.35520

Palabras clave: Biosurfactante, ramnolípidos (RL), mono-ramnolípidos, fermentador de laboratorio 7L, P. aeruginosa, síntesis de ramnolípidos, extracción, emulsificación, cromatografía, determinación de citotoxicidad

Valdés R, Aragón H, González M, Hernández D, Geada D, Goitizolo D et al. Mouse Hybridoma Cell Culture in a Protein-Free Medium Using a Bio-Mimicking Fish-Tail Disc Stirred Bioreactor. BioProcessing Journal, Volume 16, Issue: 1, Spring 2017. Pages: 51–64. Center for Genetic Engineering and Biotechnology. Cuba.
https://doi.org/10.12665/J161.Valdes

Palabras claves: Cultivo de hibridoma, CB.Hep-1 , Biomimicking fish-tail, bioreactor.

Resumen: Because the LAMBDA MINIFOR bioreactor provides good mixing of cell culture, nutrients, and gases without any damaging hydrodynamic forces by using a bio-mimicking “fish-tail“ disc stirrer, it can be successfully applied for the cultivation of bacteria and yeast, insect, plant, and mammalian cells. However, reports on its application in mouse hybridoma cell culture using protein-free media is non-existent in the scientific literature. Therefore, this study describes preliminary findings of the Lambda MINIFOR bioreactor suitability in mouse hybridoma cell culture and antibody production using the SP2/O-Ag14-CB.Hep-1 mouse hybridoma cell and the PFHM-II protein-free medium as models. [showhide type="link" more_text="Leer más" less_text="Ocultar"]Results verified 2.45 × 10⁶ viable cells/mL as the highest cell concentration, 86% as maximum cell viability, 0.0156/h as the exponential growth rate, 44 h as cell population doubling time, a stable phenotype measured by limiting dilution after 2.5 months, no antibiotic and antifoam requirements, 71.4% of IgG SDS-PAGE purity in the cell culture harvested supernatant, 38.68 ± 22.29 μg/mL, 39.23 ± 10.66 pg/cell/day, up to 99.5% of purity (sample measured by SDS-PAGE and SE-HPLC) after an IgG capture step based on protein A-Sepharose, a low pH incubation, and size-exclusion chromatography, no molecule aggregation, specificity for the CKTCTT epitope (located in the HBsAg “a” determinant), an IgG affinity constant equal to 1.11 × 10¹⁰ M^-1, and < 78 pg mouse DNA/mg of IgG. In conclusion, this study corroborated a cumulative CB.Hep-1 mAb production of 1.77 g/15 days and validated the usefulness of the Lambda MINIFOR bioreactor in mouse hybridoma cell culture in protein-free media for research applications...

Baiye Mfortaw Mbong, Victor, Jerry Ampofo-Asiama, Maarten Hertog, Annemie H Geeraerd and Bart M. Nicolai. (2017) Metabolic profiling reveals a coordinated response of isolated lamb's (Valerianella locusta, L.) lettuce cells to sugar starvation and low oxygen stress. Postharvest Biology and Technology. Volume 126, April 2017, Pages: 23-33.  Department of Biochemistry, University of Cape Coast, Ghana.
https://doi.org/10.1016/j.postharvbio.2016.12.004

Palabras claves: Células de Lechuga Lamb (Valerianella locusta L.); Carencia de azúcares; Estrés a baja concentración de O₂; Respuesta metabólica; Perfil de metabolitos; Marcaje ¹³C.

Resumen: Sugar starvation is a common phenomenon occurring in most leafy vegetables after harvest and storage. Additionally, leafy vegetables are subjected to low O₂ stress when stored in modified atmosphere conditions. In this study, the metabolism of isolated lamb’s lettuce cells was studied upon sugar starvation under O₂ stress conditions, using ¹³C labelled glucose. Fast depletions of the soluble sugars were observed, being more pronounced under aerobic conditions than under low O₂ stress conditions. Sugar starvation under aerobic conditions resulted in increased levels and decreased ¹³C label incorporation of TCA cycle intermediates and amino and fatty acids originating from glycolytic and TCA cycle pathways, compared to starving cells incubated under low O₂stress. [showhide type="link" more_text="Leer más" less_text="Ocultar"]On incubation under low O₂ stress a switch in metabolism from aerobic to fermentation metabolism was observed. Under low O₂ stress conditions, increased levels and ¹³C label incorporated in hexose phosphates, pyruvate, lactate, GABA, alanine, together with increased levels of acetaldehyde, ethanol and ethyl acetate was observed indicating fermentative metabolism was triggered.

Robusta producción de etanol celulósico a partir de bagazo de la caña de azúcar con  Saccharomyces cerevisiae ATCC 20602 en el bioreactor LAMBDA MINIFOR bajo condiciones aeróbicas y anaeróbicas con medición controlada del potencial Redox

Jabasingh, S. Anuradha, D. Lalith, M. Arun Prabhu, Abubekker Yimam, and Taye Zewdu. (2016) Catalytic conversion of sugarcane bagasse to cellulosic ethanol: TiO2 coupled nanocellulose as an effective hydrolysis enhancer. Carbohydrate Polymers (2016.Carbohydrate Polymers, Volume 136, 20 January 2016, Pages 700-709). Addis Ababa Institute of Technology, Ethiopia; Sathyabama University, India.
https://doi.org/10.1016/j.carbpol.2015.09.098

Palabras claves: Etanol celulósico, bagazo, dióxido de titanio, nanocelulosa, celulasa de Saccharomyces cerevisiae

Resumen: The present study deals with the production of cellulosic ethanol from bagasse using the synthesized TiO₂ coupled nanocellulose (NC-TiO₂) as catalyst. Aspergillus nidulans AJSU04 cellulase was used for the hydrolysis of bagasse. NC-TiO₂ at various concentrations was added to bagasse in order to enhance the yield of reducing sugars. Complex interaction between cellulase, bagasse, NC-TiO₂ and the reaction environment is thoroughly studied. A mathematical model was developed to describe the hydrolysis reaction. Ethanol production from enzymatically hydrolyzed sugarcane bagasse catalyzed with NC-TiO₂ was carried out using Saccharomyces cerevisiae ATCC 20602. The glucose release rates and ethanol concentrations were determined. Ethanol produced was found to be strongly dependent on pretreatment given, hydrolysis and fermentation conditions. The study confirmed the promising accessibility of NC-TiO₂, for enhanced glucose production rates and improved ethanol yield.

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Ménoret, S. et al. (2015) Homology-directed repair in rat zygotes using Cas9 and TALEN engineered proteins. Sci. Rep. 5, 14410; NSERM UMR 1064-ITUN; CNRS UMS3556 Nantes; CNRS UMR7196; Sorbonne Universities; University Pierre & Marie Curie; France.
https://doi.org/10.1038/srep14410

Resumen: The generation of genetically-modified organisms has been revolutionized by the development of new genome editing technologies based on the use of gene-specific nucleases, such as meganucleases, ZFNs, TALENs and CRISPRs-Cas9 systems. The most rapid and cost-effective way to generate genetically-modified animals is by microinjection of the nucleic acids encoding gene-specific nucleases into zygotes. However, the efficiency of the procedure can still be improved. In this work we aim to increase the efficiency of CRISPRs-Cas9 and TALENs homology-directed repair by using TALENs and Cas9 proteins, instead of mRNA, microinjected into rat and mouse zygotes along with long or short donor DNAs. We observed that Cas9 protein was more efficient at homology-directed repair than mRNA, while TALEN protein was less efficient than mRNA at inducing homology-directed repair. Our results indicate that the use of Cas9 protein could represent a simple and practical methodological alternative to Cas9 mRNA in the generation of genetically-modified rats and mice as well as probably some other mammals.

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Fermentación de un microorganismo modificado por ingeniería genética en el bioreactor de escala de laboratorio LAMBDA MINIFOR, para lograr una conversión eficiente de lactosa a etanol:

Pasotti, L., S. Zucca, M. Casanova, N. Politi, I. Massaiu, G. Mazzini, G. Micoli, C. Calvio, M. G. Cusella De Angelis, and P. Magni. (2015) Methods for genetic optimization of biocatalysts for biofuel production from dairy waste through synthetic biology. 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Milan, Italy, 2015, pp. 953-956.
Department of Electrical, Computer & Biomedical Engineering and Interdepartmental Research Centre for Tissue Engineering, University of Pavia, Italy.
https://doi.org/10.1109/EMBC.2015.7318521

Palabras claves: conversión lactosa a etanol; optimización de microorganismos, biología sintética, proteíanas del trigo, permeato, disposición de desechos de polutantes; optimización genética; producción de energía verde, producción de biofuel, proceso de producción de queso, desecho diario, biocatalizador.

Resumen: Whey is an abundant by-product of cheese production process and it is considered a special waste due to its high nutritional load and hypertrophic potential. Technologies for whey valorization are available. They can convert such waste into high-value products, like whey proteins. However, the remaining liquid (called permeate) is still considered as a polluting waste due to its high lactose concentration. The alcoholic fermentation of lactose into ethanol will simultaneously achieve two important goals: safe disposal of a pollutant waste and green energy production. This methodology paper illustrates the workflow carried out to design and realize an optimized microorganism that can efficiently perform the lactose-to-ethanol conversion, engineered via synthetic biology experimental and computational approaches.

El sistema de biorreactor MINIFOR de LAMBDA se utiliza como boca artificial para el cultivo de biopelículas

LA BOCA ARTIFICIAL DE DENTAID UNA REVOLUCIÓN EN INVESTIGACIÓN BUCODENTAL DENTAID EXPERTISE, PUBLICACIÓN PARA PROFESIONALES DE LA ODONTOLOGÍA, NÚM. 18. https://aprenderly.com/doc/3463742/la-boca-artificial-de-dentaid-una-revoluci%C3%B3n-en-investiga...?page=5 (2022 September 13)

Un modelo de biofilme celular de flujo con 6 especies se desarrolló y se cultivaron las bacterias en el fermentador LAMBDA MINIFOR para evaluar el desarrollo del biofilme bajo condiciones de fuerzas de corte y flujo:

Palabras claves: caries dental, cultivo en batch o lote, cultivo continuo, boca artificial, flujo celular y microcosmos.

Resumen: A dental biofilm forms a distinct environment where microorganisms live in a matrix of extracellular polysaccharides. The biofilm favors certain bacteria and creates a habitat that functions differently compared to planktonic bacteria. Reproducible model systems which help to address various questions related to biofilm formation, the process of caries development, and its prevention are needed and are continuously developed. Recent research using both batch culture, continuous culture and flow cells in caries biofilm formation is presented. The development of new techniques and equipment has led to a deeper understanding of how caries biofilms function. Biofilm models have also been used in the development of materials inhibiting secondary caries. This short review summarizes available models to study these questions.

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Cuantificación de las proteínas ribosomales (RPs) a partir de células de levaduras cultivadas en el MINIFOR y células madres embrionarias de ratón (ESC) para estudiar la estequeometría del core o núcleo de las RPs:

Slavov, N., Semrau, S., Airoldi, E., Budnik, B. & van Oudenaarden, A. (2015) Differential stoichiometry among core ribosomal proteins, arXiv:1406.0399. Jun 2015. Cell Reports 13 , 865–873 November 3, 2015. Harvard University, USA; Broad Institute of MIT and Harvard, USA and Hubrecht Institute, Netherlands .
https://doi.org/10.48550/arXiv.1406.0399

Palabras claves: Levaduras, células madres embrionarias (ESC), Proteínas ribosomales, RP, ribosomas, mRNA, espectrometría de masa, modificaciones posttraduccionales.

Resumen: Understanding the regulation and structure of ribosomes is essential to understanding protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.

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Crecimiento de cultivos de levaduras (DBY12007) en el LAMBDA MINIFOR en estado estacionario para estudiar la glicólisis aerobia y el flujo de energía

Slavov, N., Budnik, B., Schwab, D., Airoldi, E. & van Oudenaarden, A. (2014). Constant Growth Rate Can Be Supported by Decreasing Energy Flux and Increasing Aerobic Glycolysis. Cell Reports 7, 705–714, May 8, 2014.   Massachusetts Institute of Technology, USA; Harvard University, USA; Hubrecht Institute, Netherlands and Princeton University, USA.

https://doi.org/10.1016/j.celrep.2014.03.057

Palabras claves: Levaduras, glicólisis aerobia, crecimiento exponencial, consumo de O2, producción de CO2, aminoácidos, mRNAs, proteínas, modificaciones posttraduccionales, sensibilidad al estrés, coeficiente respiratorio (RQ).

Resumen: Fermenting glucose in the presence of enough oxygen to support respiration, known as aerobic glycolysis, is believed to maximize growth rate. We observed increasing aerobic glycolysis during exponential growth, suggesting additional physiological roles for aerobic glycolysis. We investigated such roles in yeast batch cultures by quantifying O₂ consumption, CO₂ production, amino acids, mRNAs, proteins, posttranslational modifications, and stress sensitivity in the course of nine doublings at constant rate. During this course, the cells support a constant biomass-production rate with decreasing rates of respiration and ATP production but also decrease their stress resistance. As the respiration rate decreases, so do the levels of enzymes catalyzing rate-determining reactions of the tricarboxylic-acid cycle (providing NADH for respiration) and of mitochondrial folate-mediated NADPH production (required for oxidative defense). The findings demonstrate that exponential growth can represent not a single metabolic/physiological state but a continuum of changing states and that aerobic glycolysis can reduce the energy demands associated with respiratory metabolism and stress survival.

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El bioreactor LAMBDA MINIFOR es usado para crecer bacterias orales (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis) bajo condiciones de plakton:

V. Blanc, S. Isabal, M. C. Sánchez, A. Llama-Palacios, D. Herrera, M. Sanz & R. León. (2014). Characterization and application of a flow system for in vitro multispecies oral biofilm formation. Periodont Res 2014; Journal of Periodontal Research.
https://doi.org/10.1111/jre.12110

Keywords: modelo de biofilme; clorhexidina; microscopía confocal laser de mapeo scanning; bacterias orales.

Resumen: Bacteria in the oral cavity grow in the form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. The aims of this study were: (i) to develop and to characterize an in-vitro biofilm model with oral bacteria growing under flow and shear conditions; and (ii) to demonstrate the usefulness of the model for evaluating the activity of three antiplaque agents.We used a bioreactor to grow the oral bacteria Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis under planktonic conditions. Biofilms were established using a modified Robbins device on hydroxyapatite (HAP) discs. Three- to 7-d-old biofilms were analysed using culture methods, scanning electron microscopy, Live/Dead staining and fluorescence in-situ hybridization (confocal laser scanning microscopy). Finally, we assessed the antimicrobial activity of three mouthrinses [0.12% chlorhexidine (CHX), 0.12% chlorhexidine and sodium fluoride (CHX+NaF) and 0.12% chlorhexidine and 0.05% cetylpyridinium chloride (CHX+CPC)] using a planktonic test (short interval–killing test) and in our 4-d biofilm model. The viable cell counts showed that each species was consistently found in the biofilms throughout the study. The architecture and cell distribution were similar to those described for biofilms in situ, with the exception of a thin layer of living cells that was found close to the HAP. The effectiveness test of the mouthwashes demonstrated that cells in biofilms showed more tolerance compared with planktonic cells. Moreover, it was observed that in 4-d biofilm formed in vitro, CHX+CPC caused significantly higher mortality compared with CHX (p = 0.003) and CHX+NaF (p < 0.001). Our results suggest that we have a highly reproducible system for multispecies oral biofilm formation and that it is a useful tool for assessing antibacterial molecules before their clinical evaluation. It also has great potential to be used in basic research on supragingival and subgingival biofilms.

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Cultivo de la microalga (Chlorella vulgaris Beyerinck) en el bioreactor de laboratorio LAMBDA MINIFOR:

Heitur, Heiko. (2014). Mikrovetika Chlorella vulgaris Beyerincki kasvatamine CO2 sidumise eesmärgil. Estonian University of Life Sciences, Estonia. PhD diss., 2014.
https://www.kik.ee/sites/default/files/Uuringud/2014/kik_lopparuanne.pdf (found 2014 Dec. 30)

Fermentación en lote, selectiva y no-selectiva de extractos de dátiles usando Saccharomyces cerevisiae (línea comercial usada en reposterías (línea silvestre), líneas glucosa selectivas ATCC 36858 y ATCC 36859) en el fermentador LAMBDA MINIFOR:

Meilana Dharma Putra, Ahmed E. Abasaeed, Mohamed A. Zeinelabdeen, Mohamed H. Gaily, Ashraf K. Sulieman. (2014). Selective fermentation of pitted dates by S. cerevisiae for the production of concentrated fructose syrups and ethanol. Journal of Physics: Conference Series 495 (2014) 012034  Chemical Engineering Department, King Saud University, Saudi Arabia.
https://doi.org/10.1088/1742-6596/495/1/012034

Palabras claves: Fermentación selectiva y no selectiva, levadura, S. cerevisiae, fructosa, etanol, dátiles, HPLC, perfil cinético, lote.

Resumen: About half of worldwide production of dates is unconsumed. Dates contain over 75 % reduced sugars (mostly glucose and fructose with nearly equal amount). Compared to the commercial Saccharomyces cerevisiae wild strain, the strains ATCC 36858 and 36859 could produce high concentration fructose syrups. The fructose fractions obtained were 95.9 and 97.4% for ATCC 36858 and 86.5 and 91.4% for ATCC 36859 at 30 and 33°C, respectively. Fructose yields higher than 90% were obtained using ATCC 36858 compared to those obtained using ATCC 36859 which were 87.3 and 66.1% at 30 and 33°C, respectively. The ethanol yield using ATCC 36858 was higher than that using ATCC 36859 by 16 and 9% at 30 and 33°C, respectively. Through this finding, the production of fructose and ethanol from date extract is a promising process. Moreover, the fructose fractions obtained here (about 90%) are much higher than those obtained with the commercial process, i.e. 55 % fructose syrups.

Crecimiento controlado de Staphylococcus aureus en varias concentraciones de BAC (cloruro de benzalconio) en el fermentador LAMBDA MINIFOR.

Palabras claves: Staphylococcus aureus, cloruro de benzalconio (BAC), fase exponencial, expresión, PCR en tiempo real, cultivo, concentración

Resumen: Conditions facilitating resistance to quaternary ammonium compounds (QACs) were investigated in Staphylococcus aureus SK982 exposed to benzalkonium chloride (BAC; a member of QACs) under various circumstances. S. aureus SK982 carrying the qacA gene encoding for resistance to QACs was grown in the presence of stable or gradually increasing concentrations of BAC, or it was exposed to this antiseptic in the exponential phase of growth. Bacteria cultivated in the highest BAC concentrations that did not retard their growth comparing to the untreated control were subjected to real-time quantitative polymerase chain reaction analysis for relative expression of the efflux genes qacA and norA. Under such conditions, S. aureus SK982 tolerated a relatively low stable concentration of BAC (1.22 mg/L) when compared with a gradually increasing antiseptic concentration (tolerance of 4.88 mg/L). However, in both cases, qacA expression was not significant. The culture exposed in the exponential phase of growth tolerated the highest concentration of BAC (9.76 mg/L) as also accompanied by significant overexpression of qacA. Expression of norA was relatively low regardless of the conditions tested. It seems that under the short-term conditions, the phase of bacterial growth is more important for the expression of BAC resistance than the capability to adapt to this antiseptic. This study provides a deeper insight into the relevance of the qac genes in conferring resistance to QACs.

Sistemas para el crecimiento de hibridomas en alta densidad y cultivos celular para la producción de anticuerpos monoclonales (mAbs) con alto rendimiento: Bioreactores de tanque agitado a escala de mesa, 1-5 L (MINIFOR - LAMBDA Laboratory Instruments).

Gary C. Howard & Matthew R. Kaser. (2013). Making and using antibodies - a practical handbook. CRC Press. Boca Raton. ISBN: 1439869081, 9781439869086. CRC Press, 2013.
https://books.google.cz/books?id=yZQiab1lxEYC (found 2013 Dec. 28)

Resumen: Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science—both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications... From the eradication of smallpox to combating cancer, antibodies present an attractive solution to a range of biomedical problems. They are relatively easy to make and use, have great flexibility in applications, and are cost effective for most labs. This volume will assist biomedical researchers and students and pave the way for future discovery of new methods for making and using antibodies for a host of applications.

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Desarrollo de modelo de biofilm (S. oralis, A. naeslundii, V. parvula, F. nucleatum, A. actinomycetemcomitans y P. gingivalis) en flujo continuo con un biorreactor LAMBDA MINIFOR 0.4L

Soto, I. S. and Alonso, M. S. (2013). Desarrollo del modelo de boca artificial en flujo continuo en el biorreactor Lambda Minifor. Universidad Complutense de Madrid Master en Ciencias Odontológicas.
https://eprints.ucm.es/id/eprint/24169/1/Desarrollo_del_Modelo_de_Boca_Artificial_en_Flujo_Cont%C3%ADnuo_en_el_biorreactor_Lambda_Minifor.pdf (2022 Juli 19)

Palabras clave: biopelícula, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, Veillonella parvula, Actinomyces naeslundii, Porphyromonas gingivalis, Streptococcus oralis

El bioreactor LAMBDA Minifor usado en estudios de laboratorio para simular las condiciones reales de la boca en modelos de boca artificial. https://t.co/px3GgGYQls pic.twitter.com/sKl1aHYhyH

— LAMBDA Instruments (@LAMBDA_Labinstr) March 28, 2022

La respuesta de estrés metabólico en cultivo de células de tomate a baja concentración de oxígeno usando el bioreactor LAMBDA MINIFOR.

Ampofo-Asiama, J., Baiye, V.M.M., Hertog, M.L.A.T.M., Waelkens, E., Geeraerd, A.H. & Nicolai, B.M. (2014). The metabolic response of cultured tomato cells to low oxygen stress. Plant Biol J, 16: 594-606. German Botanical Society and The Royal Botanical Society of the Netherlands.
https://doi.org/10.1111/plb.12094

Palabras claves: 3C marcaje; cultivo celular, estrés a baja concentración de O2; Lycopersicum esculentum; metaboloma

Resumen: The storage of fruits and vegetables under a controlled atmosphere can induce low oxygen stress, which can lead to post-harvest losses through the induction of disorders such as core breakdown and browning. To gain better understanding of the metabolic response of plant organs to low oxygen, cultured tomato cells (Lycopersicum esculentum) were used as a model system to study the metabolic stress response to low oxygen (0 and 1 kPa O₂). By adding ¹³C labelled glucose, changes in the levels of polar metabolites and their ¹³C label accumulation were quantified. Low oxygen stress altered the metabolite profile of tomato cells, with the accumulation of the intermediates of glycolysis in addition to increases in lactate and sugar alcohols. ¹³C label data showed reduced label accumulation in almost all metabolites except lactate and some sugar alcohols. The results showed that low oxygen stress in tomato cell culture activated fermentative metabolism and sugar alcohol synthesis while inhibiting the activity of the TCA cycle and the biosynthesis of metabolites whose precursors are derived from central metabolism, including fluxes to most organic acids, amino acids and sugars.

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LAMBDA MINIFOR Bioreactor utilizado para la expresión de proteína recombinante (Chemokines) en E. coli.

Kramp, Birgit. (2013). Establishing the Interaction Between the CC Chemokine Ligand 5 and the Receptors CCR1 and CCR5. Aachen, Techn. Hochsch., Diss., 2013, 2013. Print.

https://publications.rwth-aachen.de/record/231006 (found 2023 Feb. 18)

Expresión recombinante de Met-CCL5, proteasa resistente CXCL12 (S4V) y F1-CX3CL1 en E. coli para estudiar su rol en la enfermedad cardiovascular (CVD).

Projahn, Delia. (2013). Generation, Function and Therapeutic Application of Chemotactic Cytokines in Cardiovascular Diseases. Aachen, Techn. Hochsch., Diss., 2013, 2013. Print.
https://publications.rwth-aachen.de/record/229207/files/4840.pdf (found 2023 Feb. 18)

Expresión de la proteína Caf1 usando la línea Escherichia coli para estudiar la adhesión de células de mamíferos, forma y números de focos de adhesión.

Machado Roque & Ana Isabel (2013). Protein scaffolds for cell culture. Institute for Cell and Molecular Biosciences, Newcastle University.
http://hdl.handle.net/10443/1843 (found 2023 Feb. 18)

LAMBDA MINIFOR Bioreactores para el cultivo de células madres.

Shayan, N., Ebrahimi, M., Beiki, B. et al. (2012). A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells. Biotechnol Lett 34, 2125–2131 (2012).
https://doi.org/10.1007/s10529-012-0993-5

Palabras claves: Cultivo estático; suspensión celular, sangre del cordón unbical y mezclado vertical

Resumen: The proliferation and differentiation characteristics of umbilical cord blood mononuclear cells were examined in a non-rotational suspension bioreactor with a fishtail mixer. The system consisted of a glass vessel, a mixer that moved vertically, a data acquisition and control system to continuously monitor pH, temperature and dissolved O₂. The bioreactor provided superior expansion of total HSCs and not total cell number, as well as expression of stemness-related genes which followed with increasing in number of colony-forming cells during 14 days of culture compared to T -lask culture. Vertical agitation thus reduces the total cell number, which may be related to increased shear stress, but has no effect on HSC function.

Producción efectiva de biobutanol a partir de desechos agrícolas.

Mežule, L., Tihomirova, K., Neščerecka, A., Juhna, T. Latvian (2012). Biobutanol Production from Agricultural Waste: A Simple Approach for Pre-Treatment and Hydrolysis. Journal of Chemistry, 2012, 51, No. 4. pp.407-414. Riga Technical University, Latvia.
https://ortus.rtu.lv/science/en/publications/16169-Biobutanol+Production+from+Agricultural+Waste%3A+A+Simple+Approach+for+Pre-Treatment+and+Hydrolysis (found 2012 Dec. 30)

Palabras claves: biofuel, biobutanol, desechos agrícolas, hidrósis

Resumen: One of the main concerns regarding extensive production of bio-butanol has been associated with the high costs of the substrate (preparation of  fermentable sugars) and the relatively low tolerance of Clostridia to butanol. In this study a simple, mild approach was tested to obtain fermentable sugars from agricultural waste. Giant hogweed and hay was pre-treated with simple boiling and enzymatically hydrolysed. The results demonstrated that after adaptation of the genus Clostridium bacteria to the new substrate, the growth kinetics and sugar consumption of these bacteria we are similar to the ones obtained in traditional culture media.

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Iva Burešová & Luděk Hřivna (2011). Effect of wheat gluten proteins on bioethanol yield from grain. Applied Energy, Volume 88, Issue 4, 2011, Pages 1205-1210, ISSN 0306-2619. Agrotest Fyto, Ltd., Kroměříž, Czech Republic; Mendel University in Brno, Brno, Czech Republic
https://doi.org/10.1016/j.apenergy.2010.10.036

Palabras claves: Bioetanol; Triticale; trigo; Gluten; Proteéna

Resumen: Bioethanol can be used as motor fuel and/or as a gasoline enhancer. A high yield feedstock for bioethanol production is cereal grain. Cereal grains containing less gluten proteins (glutenin and gliadin), but high starch, are favoured by distillers because they increase the bioethanol conversion. The direct effect of wheat gluten proteins on bioethanol yield was studied on triticale grain. Examined triticale Presto 1R.1D5+10-2 and Presto Valdy were developed by introducing selected segments of wheat chromosome 1D into triticale chromosome 1R. Even if the samples analysed in this study do not afford to make definitive assumptions, it can be noticed that in analysed cases the presence of gliadin had more significant effect on investigated parameters than the presence of glutenin. Despite the presence of glutenin subunits did not significantly decrease the investigated parameters – specific weight, Hagberg falling number and starch content in grain met the requirements for grain for bioethanol production – protein content was higher than is optimal. The fermentation experiments demonstrated good bioethanol yields but depression in grain yields caused by the presence of wheat gliadin and glutenin decreased the energy balance of Presto Valdy and Presto 1R.1D5+10-2.

Fermentación anaeróbica del componente de glucosa en los extractos de dátiles por la levadura Saccharomyces cerevisiae.

Mohamed H. Gaily, Basheir M. Elhassan, Ahmed E Abasaeed & Saeed M. Al-Zahrani. (2010). A Direct Process for the Production of High Fructose Syrups from Dates Extracts. International Journal of Food Engineering.  Volume 6, Issue 3, ISSN (Online) 1556-3758 King Saud University, Saudi Arabia; University of Khartoum, Sudan
https://doi.org/10.2202/1556-3758.1879

Palabras claves: dátiles, fructosa, glucosa, etanol, fermentación, S. Cerevisiae, levadura, mesófilo, lote

Resumen: A process comprising of extraction, fermentation, separation and decolorization for the production of high fructose syrups and/or ethanol from dates' extract was developed. In this paper, selective fermentation of the glucose component in dates' extract by Saccharomyces cerevisiae was employed to tailor the composition of the final product (fructose- or ethanol-rich). Over 90% of dates’ sugar was extracted by water. The fermentation experiments were conducted at 30 °C and 120 rpm. The commonly marketed 55% fructose syrup was obtained at two operating conditions, i.e., low (<50%) and high (>92%) sugar conversions. Maximum fructose concentrations (77.2-78.2%) were obtained at moderately high sugar conversions (74-82%). Decolorization by activated carbon for the fructose-rich syrup resulted in 87% of color removal. Preliminary economic analysis revealed the viability of this process.

Park, M. -O., Mizutani, T. & Jones, P. R. (2007). Glyceraldehyde-3-Phosphate Ferredoxin Oxidoreductase from Methanococcus maripaludis. Journal of Bacteriology, Tokyo, Japan. Research and Development Division, Fujirebio, Inc., 51 Komiya-cho, Hachioji-shi, Tokyo 192-0031, Japan.
https://doi.org/10.1128/JB.00828-07

Resumen: The genome sequence of the non-sugar-assimilating mesophile Methanococcus maripaludis contains three genes encoding enzymes: a nonphosphorylating NADP⁺-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPN), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and glyceraldehyde-3-phosphate ferredoxin oxidoreductase (GAPOR); all these enzymes are potentially capable of catalyzing glyceraldehyde-3-phosphate (G3P) metabolism. GAPOR, whose homologs have been found mainly in archaea, catalyzes the reduction of ferredoxin coupled with oxidation of G3P. GAPOR has previously been isolated and characterized only from a sugar-assimilating hyperthermophile, Pyrococcus furiosus (GAPOR_Pf), and contains the rare metal tungsten as an irreplaceable cofactor. Active recombinant M. maripaludis GAPOR (GAPOR_Mm) was purified from Escherichia coli grown in minimal medium containing 100 μM sodium molybdate. In contrast, GAPOR_Mm obtained from cells grown in medium containing tungsten (W) and W and molybdenum (Mo) or in medium without added W and Mo did not display any activity. Activity and transcript analysis of putative G3P-metabolizing enzymes and corresponding genes were performed with M. maripaludis cultured under autotrophic conditions in chemically defined medium. The activity of GAPOR_Mm was constitutive throughout the culture period and exceeded that of GAPDH at all time points. As GAPDH activity was detected in only the gluconeogenic direction and GAPN activity was completely absent, only GAPOR_Mm catalyzes oxidation of G3P in M. maripaludis. Recombinant GAPOR_Mm is posttranscriptionally regulated as it exhibits pronounced and irreversible substrate inhibition and is completely inhibited by 1 μM ATP. With support from flux balance analysis, it is concluded that the major physiological role of GAPOR_Mm in M. maripaludis most likely involves only nonoptimal growth conditions.

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pH y temperatura fueron registrados de forma continua con el LAMBDA MINIFOR y el SIAM software.

Chaignon, P., Cortial, S., Guerineau, V., Adeline, M.-T., Giannotti, C., Fan, G. & Ouazzani, J. (2005). Photochemical Reactivity of Trifluoromethyl Aromatic Amines: The Example of 3,5-diamino-trifluoromethyl-benzene (3,5-DABTF). Photochemistry and Photobiology, 81: 1539-1543. Institut de Chimie des Substances Naturelles, C.N.R.S, Avenue de la Terrasse, 91198, Gif-sur-Yvette Cedex. France.
https://doi.org/10.1562/2005-08-03-RA-637

Resumen: This work presents the application of an on-line photoreactor to the detection of 3,5-diamino-trifluoromethyl-benzene (3,sDABTF) in aqueous solutions. When irradiated at 310 nm, this compound is defluorinated to 3,s-diaminobenzoic acid by a nucleophilic substitution of the fluoride by water. Concomitantly, defluorination intermediates polymerize through amide bonds to give dark-colored compounds. We take advantage of the photocatalyzed defluorination and the subsequent decrease in pH to develop an original and specific photoreactor. Continuous recording of pH and temperature in the outlet of the reactor by a dual electrode gives us an opportunity to optimize the system. In the photoreactor, 3,5-DABTF is immediately and totally transformed as attested by the rapid drop of the flowing solution pH from 6.2 to 3.2 and the chromatographic analysis of the outgoing solutions. The detection remains effective from 1 to 1000 parts per million.

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Lehky, P. (2003). Bioreactors - New Solutions for Old Problems. Proceedings of the 1st International Congress on Bioreactor Technology in Cell, Tissue Culture and Biomedical Applications: Tampere, Finland, 14-18 July, 2003. ISBN: 9529930208, 9789529930203.

https://www.bioreactors.eu/files/bioreactor/minifor-bioreactors-article.pdf (found 2023 Feb. 18)

Palabras claves: bioreactor, fermentador, cultivo celular, electrodo DO, caudal del flujo de gas, estación de gases.

Resumen: A major effort has been invested in making bioreactors more efficient. The goal has been achieved in several steps. Firstly, a replacement of the traditional rotation movement by an oscillating up and down movement of stirring discs driven by an electromagnet yields several important advantages. A soft, whole volume movement of the medium results in optimal gas distribution throughout the medium and is also advantageous for cell growth. No vortex is formed and the elimination of baffles simplifies the construction of the bioreactor. A single silicone membrane efficiently replaces traditional seals and assures perfect sterility. In the case of extremely sensitive cells gas distribution tubing can be wound on a spiral fixed to the axis. The up and down movement facilitates the gas transfer and simultaneously provides a gentle movement of the medium. Secondly, a new heat radiation system has also been introduced. A heating spiral in a gilded parabolic reflector has been placed under the bottom of the vessel. The IR rays efficiently heat up the culture without overheating it at any volume of medium. Several innovations allow the construction of high quality bioreactors at lower cost.

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