OUTGAS / EXIT GAS MEASUREMENT: % CO2, % O2, % CH4

LAMBDA Laboratory Instruments offers the following outgas/exit gas measurement units for bench-top bioreactor systems beside off total flow measurements:

LAMBDA CARBOMETER measures CO2 [%]

LAMBDA CARBOMETER is a module to measure the concentration of carbon dioxide in a gas flow.

Connected to the outgas line of the bench-top bioreactor system, LAMBDA CARBOMETER measures the concentration of CO2 in the outgas/exit gas of the fermentation.

Technical specification of LAMBDA CARBOMETER

  • CO2 concentration: 0 – 9.99% or 10.0 – 99.9%
  • Accuracy: ±3%
  • Universal power supply: 12V/6W
  • Dimension: 105 x 80 x 170 mm (W x H x D)
  • Weight: 600 g
  • RS communication
  • CE conform

Leaflet: www.lambda-instruments.com/carbometer/#documentation

 

 

LAMBDA OXYMETER measures O2 [%]

LAMBDA OXYMETER is a module to measure the concentration of the oxygen in a gas flow.

Connected to the outgas line of the bench-top bioreactor system, LAMBDA OXYMETER measures the concentration of the O2 in the outgas/exit gas of the fermentation.

Technical specification of LAMBDA OXYMETER

  • O2concentration: 0 – 9.99% or 10.0 – 25%
  • O2partial pressure (ppO2): 0 – 250 mBar
  • Total pressure: 500 – 1200 mBar
  • Temperature: 0 – 60 ⁰C
  • Accuracy: ±2%
  • Universal power supply: 12V/6W
  • Dimension: 105 x 80 x 170 mm (W x H x D)
  • Weight: 600 g
  • RS communication
  • CE conform

Leaflet: www.lambda-instruments.com/oxymeter/#documentation

 

 

 

LAMBDA METHAMETER measures CH4 [%]

LAMBDA METHAMETER is a module to measure the concentration of the methane in a gas flow.

 

Connected to the outgas line of the bench-top bioreactor system, LAMBDA METHAMETER measures the concentration of CH4 in the outgas/exit gas of the fermentation.

Technical specification of LAMBDA METHAMETER

  • CH4concentration: 0 – 9.99% or 10.0 – 99.9%
  • Universal power supply: 12V/6W
  • Dimension: 105 x 80 x 170 mm (W x H x D)
  • Weight: 600 g
  • RS communication
  • CE conform

Leaflet: www.lambda-instruments.com/methameter/#documentation

Automatic calculation of RQ, OUR, CPR

SIAM automation PC software allows the possibility to calculate the RQ (respiratory quotient or respiratory coefficient) from

  • OUR (Oxygen uptake rate) and
  • CPR (carbon dioxide production rate),

by values obtained from LAMBDA OXYMETER and LAMBDA CARBOMETER measurements during the fermentation process.

 

How is the respiration quotient (RQ) calculated?

Respiratory quotient is defined as the number of moles of CO2 produced per mole of O2 consumed.

 

 

where,
RQ = Respiratory quotient or respiratory coefficient
CPR = Carbon dioxide production rate
OUR = Oxygen transfer rate

The RQ provides useful information about the metabolic activity and physiological conditions of the microorganism based on the relation between carbon dioxide production (CPR) and oxygen consumption (OUR) during fermentation process.

Oxygen transfer rate (OTR)

Monitoring the oxygen transfer rate (OTR) of aerobic animal cell, plant cell, fungal, yeast cell and bacterial culture reveals important information about the physiological state of the culture like substrate or oxygen limitation, diauxic growth and product inhibitions.

Carbon dioxide transfer rate (CTR)

The carbon dioxide transfer rate (CTR) and respiratory quotient (RQ) obtained can be used to predict the biomass concentration, evaluate energetic efficiencies of growth, monitoring growth yields and controlling fed batch processes